Intellectual Merit: This research developed a virus-host model system for both biogeochemical and molecular studies of an environmentally relevant heterotrophic bacterioplankton lineage. The work fundamentally advanced our understanding of host-virus interactions at the metabolic level, provided unprecedented insight into the flux of C and N related released by phage-mediated lysis into natural seawater communities and highlighted the extent of genetic diversity harbored by roseophage. This past work did not explicitly focus on elucidating the underlying genetic mechanisms dictating cellular physiologies, but sets the stage for the current proposal, as is detailed in the preliminary data section. Broader impacts: This award supported the cross-disciplinary training of 8 graduate students (4 Microbiology, 4 Chemistry). Four of these students (3 PhD, 1 MSc) have completed their degrees. The PhD students took postdoctoral positions at Cornell, Duke and Vanderbilt; the MSc Chemistry grad is currently employed by Aegis Sciences Corp. Additionally, this award also supported the training of 8 undergraduate students, including three Microbiology REU summer students and one UTK Computer Science student. All of the graduate students and many of the undergraduates are co-authors on papers, which are indicated with a symbol in the reference section. All of these students presented their work at local, national or international symposia or conferences. The specific contributions of several of these students are highlighted in the preliminary data section of this proposal. Products to date: To date, this project has supported the publication of 19 journal publications and 1 book chapter. (detailed in the Key Outcomes and Products sections). Two additional manuscripts are in minor revision and two more manuscripts (details of which are in the preliminary data section) are near submission ready. Furthermore, from 2012-2014, Buchan was part of an NSF-sponsored National Institute of Mathematical and Biological Synthesis (NIMBioS) working group entitled Ocean Viral Dynamics. This working group was comprised of aquatic viral ecologists, microbiologists and theorists with expertise in either viral ecology or large-scale ecosystem models. The goal of this working group was to develop analytical approaches to quantify viral effects on biogeochemical cycles, principally C, N and P and cumulated in 3 publications. Finally, the research conducted under this award was included in the dissertations/thesis of 3 PhD students and 1 MSc student. These dissertations/thesis are available for public download from the University of TennesseeÆs Trace System. Our principle mechanism for sharing results from our work in a format that is accessible to the scientific community is via publication. We follow scientific community and journal guidelines on deposition of nucleic acid data to NCBI and the accession numbers to access all gene data arising from this work are: HQ317387, HQ332142, HQ317384, JPOY00000000. These accession numbers are also documented in publications describing the genetic characterization of the host and strain. For the metabolomics data, there is not yet a public repository for these types of data, thus we try to make these data easily obtainable for other researchers. For example, all of the raw metabolomics data (mass spectra peak area) were provided as supplemental data to the primary article and are available for download from the publishing journal (International Society for Microbial Ecology). For those metabolomics data that have not yet been published, we will plan to do the same thing with these data as the manuscript is submitted, within the next 2-4 months (by August 2016). In addition, we participated in a research cruise in July 2013, which was supported by another NSF OCE award to Erik Zinser (UTK) and the ancillary data are available at: http://www.bco-dmo.org/deployment/515629. We have also established a BCO-DMO webpage for o...
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