Experimental design
A flow chart of the experimental design is shown in Figure 1, including collection and incubation of the natural bloom in a short-term (2 week) pCO2 experiment, isolation of cells of all four species from each pCO2 treatment, conditioning of the isolates at the pCO2 from which they were isolated for one year, and recombining isolates into artificial communities to compete for 2-week periods following 4, 8 and 12 months of conditioning. Growth rates of each species were assessed during conditioning in unialgal cultures at the 8-month timepoint, as well as in mixed communities during the initial bloom experiment and the 12-month artificial community experiment. “Switch” competition experiments in which clones conditioned at each pCO2 were competed in artificial communities at the other two pCO2 levels were also performed after 12-months. The switch competition data are in a separate data set.
Caron, D., Fu, F., Hutchins, D. A., Schnetzer, A. (2012) Data comparing short vs. long term acclimation of a dinoflagellate community from work completed in the Hutchins Laboratory, University of Southern California from 2009-2010. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2012-11-20) Version Date 2012-11-20 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3796 [access date]
Terms of Use
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