Dataset: Nutrients, targeted proteomics, and pigments from the METZYME cruise (KM1128)

Final with updates expectedVersion 24 May 2018 (2018-05-24)Dataset Type:Cruise Results

Principal Investigator: Mak A. Saito (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Program: U.S. GEOTRACES (U.S. GEOTRACES)

Project: Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients (GPc03) (MetZyme)

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This dataset includes data from 3 sampling systems:
- Macronutrients from the trace metal rosette (TMR); analyzed by Joe Jennings at OSU.
- Targeted metaproteomics from the McLane pumps; analyzed by triple quadrupole mass spectrometry calibrated with stable isotope labeled peptides.
- Pigments from a combination of CTD and TMR samples; determined by HPLC; analyzed by Jack DiTullio, University of Charleston. Note that pigments were collected by both CTD and TMR due to a loss of the CTD as station 2/3.

Samples were collected during the KM1128 METZYME research expedition (Metals and Enzymes in the Pacific) on the R/V Kilo Moana October 1-25, 2011 from Oahu, Hawaii to Apia, Samoa.

01 Aug 2017: dataset was revised to include columns for date/time and cast numbers for each sampling system.

Related publications:
Saito, M. A., M. R. McIlvin, D. M. Moran, T. J. Goepfert, G. R. DiTullio, A. F. Post, and C. H. Lamborg. 2014. Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers. Science 345:1173-1177. DOI: 10.1126/science.1256450

Saito, M. A.; Dorsk, A.; Post, A. F.; McIlvin, M.; Rappé, M. S.; DiTullio, G.; Moran, D. 2015.  Needles in the Blue Sea: Sub‐Species Specificity in Targeted Protein Biomarker Analyses Within the Vast Oceanic Microbial Metaproteome. Proteomics. DOI: 10.1002/pmic.201400630


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