Dataset: Trichodesmium nutrient-limited proteomes in 380 and 750 uatm CO2 from experiments conducted at the University of Southern California from 2011-2013 (HiCO2_AdaptCyano project)

Final no updates expectedVersion 2 (2018-03-15)Dataset Type:experimental

Principal Investigator, Contact: David A. Hutchins (University of Southern California)

Co-Principal Investigator: Mak A. Saito (Woods Hole Oceanographic Institution)

Co-Principal Investigator: Eric A. Webb (University of Southern California)

BCO-DMO Data Manager: Ms Dicky Allison (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)


Project: Adaptation of key N2-fixing cyanobacteria to changing CO2 (HiCO2_AdaptCyano)

Trichodesmium erythraeum was grown in biological triplicate under two CO2 regimes (380 and 750 uatm pCO2) and four different nutrient regimes (replete, phosphorus-limited,iron-limited, iron/phosphorus colimited).  

Raw sequence reads from the Trichodesmium erythraeum used in this experiment can be accessed at The National Center for Biotechnology Information (NCBI) under the BioProject PRJNA312342

Protein sequences can be accessed through NCBI BioProject PRJNA318.

The fasta file IMG_Trichodesmium_IMS101_genes.fasta (5.4 MB) contains all of the Integrated Microbial Genomes (IMG)-annotated genes in the Trichodesmium erythraeum IMS101 genome.  More information can be found at the IMG Trichodesmium genome portal, "genes page" and IMG Genome ID 637000329 page.

Raw spectra are available as dataset PXD010515 in the PRIDE proteome-exchange repository (doi: 10.6019/PXD010515).

These results were published in:
* Walworth et al., 2016a
* Walworth et al., 2016b
* Walworth et al., 2018

Related dataset: Trichodesmium physiology and cell growth


Related Datasets

Related Research

Dataset: https://doi.org/10.6019/PXD010515
ProteomeXchange dataset PXD010515. Trichodesmium LC-MSMS iron and phosphorous co-limitation in the ocean. doi:10.6019/pxd010515

Related Publications

Methods, Results

Walworth, N. G., Fu, F.-X., Webb, E. A., Saito, M. A., Moran, D., Mcllvin, M. R., … Hutchins, D. A. (2016b). Mechanisms of increased Trichodesmium fitness under iron and phosphorus co-limitation in the present and future ocean. Nature Communications, 7, 12081. doi:10.1038/ncomms12081
Methods, Results

Walworth, N. G., Lee, M. D., Fu, F.-X., Hutchins, D. A., & Webb, E. A. (2016a). Molecular and physiological evidence of genetic assimilation to high CO2in the marine nitrogen fixerTrichodesmium. Proceedings of the National Academy of Sciences, 113(47), E7367–E7374. doi:10.1073/pnas.1605202113
Methods, Results

Walworth, N. G., Lee, M. D., Suffridge, C., Qu, P., Fu, F.-X., Saito, M. A., … Hutchins, D. A. (2018). Functional Genomics and Phylogenetic Evidence Suggest Genus-Wide Cobalamin Production by the Globally Distributed Marine Nitrogen Fixer Trichodesmium. Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.00189