Dataset: Proteomics Colwellia psychrerythraea strain 34H at subzero temperatures.

Version (2017-11-13)Dataset Type:experimental

Principal Investigator: Brook L. Nunn (University of Washington)

Co-Principal Investigator: Karen Junge (University of Washington)

BCO-DMO Data Manager: Megan Switzer (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Linking geochemistry and proteomics to reveal the impact of bacteria on protein cycling in the ocean (Bacterial Recyclers)

Bacteria was collected from cultures, filtered, and lysed, digested and analyzed using proteomic mass spectrometry.

Data are available for download at the EBI PRIDE Archive and at the Chorus Project Archive. 

EBI PRIDE
Homepage: http://www.ebi.ac.uk/pride/archive
Project URL: http://www.ebi.ac.uk/pride/archive/projects/PXD006274
Data URL: http://www.ebi.ac.uk/pride/archive/projects/PXD006274/files

Chorus Project
Data URL: https://chorusproject.org/anonymous/download/experiment/-1211384729456854716

Data were published in Nunn et al (2015).


Related Datasets

No Related Datasets

Related Publications

Results

Nunn, B. L., Slattery, K. V., Cameron, K. A., Timmins-Schiffman, E., & Junge, K. (2015). Proteomics of Colwellia psychrerythraeaat subzero temperatures - a life with limited movement, flexible membranes and vital DNA repair. Environmental Microbiology, 17(7), 2319–2335. doi:10.1111/1462-2920.12691
Methods

MacLean, B., Tomazela, D. M., Shulman, N., Chambers, M., Finney, G. L., Frewen, B., … MacCoss, M. J. (2010). Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics, 26(7), 966–968. doi:10.1093/bioinformatics/btq054