Dataset: Shotgun Metaproteomics of Bering Strait surface water and Chukchi Sea bottom water.

Location:  Water samples were collected in August of 2013 from the Bering Strait (BSt) chlorophyll maximum layer (7 m depth, 65°43.44″ N, 168°57.42″ W) and from the more northern Chukchi Sea (CS) bottom waters (55.5 m depth, 72°47.624″ N, 16°53.89″ W) using a 24-bottle CTD (conductivity, temperature, and depth) rosette (10 L General Oceanics Niskin X). The measurement of integrated water column chlorophyll was 226.88 mg/m2 at station BSt and 2.64 mg/m2 at station CS.

Water was collected on ship, filtered, and bacterial fractions were lysed, digested and analyzed using proteomic mass spectrometry.

Cruise = BEST Cruise 2013

Data are available for download at the EBI PRIDE Archive. Project number = PXD006472.

Homepage: http://www.ebi.ac.uk/pride/archive
Project URL: http://www.ebi.ac.uk/pride/archive/projects/PXD006472
Data URL: http://www.ebi.ac.uk/pride/archive/projects/PXD006472/files

Data are published in May, D.H., Timmins-Schiffman, E., Mikan, M.P., Harvey, H.R., Borenstein, E., Nunn, B.L., Noble, W.S. (2016) An alignment-free "metapeptide" strategy for metaproteomic characterization of microbiome samples using shotgun metagenomic sequencing. Journal of Proteome Research 15, 2697-2705. DOI: 10.1021/acs.jproteome.6b00239