Dataset: Proteomic data of dilution series of bacteria and diatoms using MS.

Version (2017-11-13)Dataset Type:experimental

Principal Investigator: Brook L. Nunn (University of Washington)

Co-Principal Investigator: Rodger Harvey (Old Dominion University)

Co-Principal Investigator: William S. Noble (University of Washington)

BCO-DMO Data Manager: Megan Switzer (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Proteins as functional biomarkers: integrating organic characterization with proteomics to track routes for carbon and nitrogen recycling and preservation (Proteins as Biomarkers)

To mimic a complex marine sample, a dilution series of R. pomeroyi and T. pseudonana was created at different cellular ratios. These mixtures were filtered and proteins were extracted from the filter for tryptic digestion and LC-MS/MS analysis.

Biological fractions were lysed, digested and analyzed using proteomic mass spectrometry.

Data are available for download at the EBI PRIDE Archive.
Homepage: https://www.ebi.ac.uk/pride/archive
Project URL: https://www.ebi.ac.uk/pride/archive/projects/PXD004758
Data URL: https://www.ebi.ac.uk/pride/archive/projects/PXD004758/files


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