Dataset: Chlorophyll measurements from the MesoHux mesocosm experiment held in May 2017

Final no updates expectedDOI: 10.26008/1912/bco-dmo.748471.1Version 1 (2018-09-14)Dataset Type:experimental

Principal Investigator: Matthew D. Johnson (Woods Hole Oceanographic Institution)

Co-Principal Investigator: Kay D. Bidle (Rutgers University)

Co-Principal Investigator: Elizabeth Harvey (University of Georgia)

BCO-DMO Data Manager: Mathew Biddle (Woods Hole Oceanographic Institution)


Project: Quantifying competing loss rates of viral lysis and microzooplankton grazing on Emiliania huxleyi mortality (E huxleyi Mortality)


Abstract

Chlorophyll measurements from the MesoHux mesocosm experiment held in May 2017.

Water samples for chlorophyll extraction were collected from the mesocosms via a 5 L Niskin. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.

Mesocosm treatments are as follows:
P-limited: N:P added in a 60:1 ratio during the first 3 days of the experiment, no shading
Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading
Shaded: N:P added in a 16:1 ratio during the first 3 days of the experiment, top shaded of the mesocosm added on May 20, 2017
Ambient: no nutrients added, no shading
Samples were taken from either 1m, 5m, or from sediment from settling cones. Samples were noted in the Sample Depth (m) column.


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