File(s) | Type | Description | Action |
---|---|---|---|
tep.csv (5.96 KB) | Comma Separated Values (.csv) | Primary data file for dataset ID 748500 | Download |
Transparent exopolymer particle (TEP) measurements from the MesoHux mesocosm experiment held in May 2017.
Water samples (75-250 mL) were gently (<150 mbar) filtered through 25 mm, 0.40 μm polycarbonate filters (Millipore, Isopore, HTTP02500). Post-filtration, filters were stained with 500 μL of a solution of 0.04% Alcian Blue (AB) and 0.06% acetic acid (pH 2.5) and were frozen at -20°C. For extraction, filters were immersed in sulfuric acid (80%) for 2 h and their absorbance was then measured spectrophotometrically (Agilent 8453 UV-visible spectrophotometer) at fixed wavelength of 787 nm. The AB-dye was calibrated using methods found in Bittar et al. (2018), where AB was used to stain known concentrations of xanthan gum (XG, Sigma-Aldrich). Two separate AB solutions (f factors 169 and 129) were used during the Bergen experiment. TEP concentrations (μg xanthan gum (Xg) equivalents L-1; μg XG eq. L-1) were calculated according to Passow and Alldredge (1995).
Mesocosm treatments are as follows:
P-limited: N:P added in a 60:1 ratio during the first 3 days of the experiment, no shading
Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading
Shaded: N:P added in a 16:1 ratio during the first 3 days of the experiment, top shaded of the mesocosm added on May 20, 2017
Ambient: no nutrients added, no shading
Johnson, M. D., Bidle, K. D., Harvey, E. (2021) Transparent exopolymer particle (TEP) measurements from the MesoHux mesocosm experiment held in May 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-09-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.748500.1 [access date]
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