Samples for quantification of B-vitamins B1, B7, CB12 and Methionine were collected at a six of depths within the euphotic zone (5-250m). Seawater was collected from each CTD depth using Niskin bottles and immediately filtered. Particulate samples for chlorophyll quantifications were collected using in-line 0.2um, 3µm and 10um pore-size filters and a peristaltic pump (flow rate < 50 ml per minute), transferred into sterile cryovials and were immediately stored at -80 degrees C until analysis. Pigments were extracted from the filters in 3 mL of methanol, BHT(butylated hydroxytoluene) was added and placed in a -20 degrees C freezer overnight. For chlorophyll-a measurements, 100 microliters of the pigment extraction were diluted in acetone (50x dilution) and analyzed using a Turner 10AU fluorometer.
B-vitamins and Methionine samples were analyzed as in Suffridge et al. 2017. Two liters of seawater were filtered through um pore-size filters and then preconcentrated using a C18 resin (HF Bondesil (Agilent Technologies) and analyzed by liquid chromatography/triple mass spectrometry (LC/MS/MS/MS). The LCMS system consists of a ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography system. The LC system used a stable- bond C18 reversed-phase column (DiscoveryHSC18 10cm × 2.1mm,5 μm column, Supelco Analytical) with a100 uL sample loop. In order to increase the sensitivity and precision, theLC/MS was run in full-loop mode (100 ul/injection).
Bacterial cell counts were heterotrophic prokaryotes were enumerated by flow-cytometry (Becton–Dickinson FACScalibur) (Gasol & del Giorgio, 2000).
Sanudo-Wilhelmy, S. A., Hutchins, D. A. (2019) Vitamin and methionine quantifications from SPOT cruises during different months from from March to December 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2019-02-06 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/753883 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.