Confocal pH measurements were made using ratiometric analysis of two forms of the dual emission pH-sensitive dye SNARF-1 (Invitrogen) according to methods published previously (Venn et al. 2011; 2015).

See Comeau et al. (2017) for a detailed overview of the methodology.

Location:  
Centre Scientifique de Monaco


Related Datasets

IsRelatedTo

Dataset: Comeau 2017 PRSB: TA anomaly calcification
Relationship Description: Data from the same experiment.
Carpenter, R., Edmunds, P. J., Comeau, S. (2019) Comeau 2017 PRSB: TA anomaly calcification. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-02-01 http://lod.bco-dmo.org/id/dataset/754766
IsRelatedTo

Dataset: Comeau 2017 PRSB: Buoyant weight calcification
Relationship Description: Data from the same experiment.
Carpenter, R., Edmunds, P. J., Comeau, S. (2020) Comeau 2017 PRSB: Buoyant weight calcification. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-12-02 http://lod.bco-dmo.org/id/dataset/754794
IsRelatedTo

Dataset: Comeau 2017 PRSB: Carbonate chemistry
Relationship Description: Data from the same experiment.
Carpenter, R., Edmunds, P. J., Comeau, S. (2020) Comeau 2017 PRSB: Carbonate chemistry. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-12-02 http://lod.bco-dmo.org/id/dataset/754818
IsRelatedTo

Dataset: Comeau 2017 PRSB: Photosynthesis
Relationship Description: Data from the same experiment.
Carpenter, R., Edmunds, P. J., Comeau, S. (2020) Comeau 2017 PRSB: Photosynthesis. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-12-02 http://lod.bco-dmo.org/id/dataset/754782

Related Publications

Results

Comeau, S., Tambutté, E., Carpenter, R. C., Edmunds, P. J., Evensen, N. R., Allemand, D., … Venn, A. A. (2017). Coral calcifying fluid pH is modulated by seawater carbonate chemistry not solely seawater pH. Proceedings of the Royal Society B: Biological Sciences, 284(1847), 20161669. doi:10.1098/rspb.2016.1669
Methods

Venn, A. A., Tambutté, E., Holcomb, M., Laurent, J., Allemand, D., & Tambutté, S. (2013). Impact of seawater acidification on pH at the tissue–skeleton interface and calcification in reef corals. Proceedings of the National Academy of Sciences, 110(5), 1634-1639.
Methods

Venn, A., Tambutté, E., Holcomb, M., Allemand, D., & Tambutté, S. (2011). Live Tissue Imaging Shows Reef Corals Elevate pH under Their Calcifying Tissue Relative to Seawater. PLoS ONE, 6(5), e20013. doi:10.1371/journal.pone.0020013