Turbulent microstructure profiles were collected near Milwaukee, WI, during 2017 (August 1 - August 16), 2018 (April 5 – April 20), and 2019 (May 13 - May 14) at a 55m depth site. Microstructure measurements were collected using an RSI MicroCTD (Rockland Scientific International, Inc.), which measures velocity shear, temperature, turbidity, and chlorophyll concentrations. High resolution velocity shear (512 Hz) measurements were used to estimate turbulent kinetic energy dissipation throughout the water column. Microstructure profiles were collected during fair weather conditions on each day of sampling.