Dataset: Dissolved organic matter (DOM) and base-extracted particulate organic matter (BEPOM) collected from a plankton senescence experiment from water samples offshore of North Carolina

Final no updates expectedDOI: 10.1575/1912/bco-dmo.773802.1Version 1 (2019-08-01)Dataset Type:experimental

Principal Investigator: Chris Osburn (North Carolina State University)

Co-Principal Investigator: Thomas Bianchi (University of Florida)

Co-Principal Investigator: Astrid Schnetzer (North Carolina State University)

Co-Principal Investigator: Kai Ziervogel (University of New Hampshire)

BCO-DMO Data Manager: Mathew Biddle (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Planktonic Sources of Chromophoric Dissolved Organic Matter in Seawater (PlankDOM)


Abstract

A mixed assemblage of natural phytoplankton community collected from waters offshore of North Carolina were used to create planktonic dissolved organic matter (DOM) and particulate organic matter (POM). The latter was extracted with 0.1 M NaOH to create base-extracted POM (BEPOM). Methods are reported in Kinsey et al. (2018) and Osburn et al. (2019). The medium used was: Natural Assemblage 2x filtered North Atlantic Surface water with f/2 nutrients; Whole water experiment - unfiltered North Atla...

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A mixed assemblage of natural phytoplankton community collected from waters offshore of North Carolina were used to create planktonic dissolved organic matter (DOM) and particulate organic matter (POM). The latter was extracted with 0.1 M NaOH to create base-extracted POM (BEPOM). Methods are reported in Kinsey et al. (2018) and Osburn et al. (2019). 
The medium used was: Natural Assemblage 2x filtered North Atlantic Surface water with f/2 nutrients; Whole water experiment - unfiltered North Atlantic Surface water with f/20 nutrients Irradiance ~70-100 umol photon m-2 s-1 (cool white bulbs); on roller table; diel cycle, 19 °C 

Controls:  0.2 um Filtered Sargasso seawater amended with f/2 nutrients; NASW (or WW) unfiltered Sargasso Seawater amended with f/20 nutrients; NASW: Sargasso seawater amended with plankton tow sample and f/2 nutrients.  All samples were in 1.9 L bottles with minimal headspace.


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Results

Osburn, C. L., Kinsey, J. D., Bianchi, T. S., & Shields, M. R. (2019). Formation of planktonic chromophoric dissolved organic matter in the ocean. Marine Chemistry, 209, 1–13. doi:10.1016/j.marchem.2018.11.010
Methods

Green, S. A., & Blough, N. V. (1994). Optical absorption and fluorescence properties of chromophoric dissolved organic matter in natural waters. Limnology and Oceanography, 39(8), 1903–1916. doi:10.4319/lo.1994.39.8.1903
Methods

Kinsey, J. D., Corradino, G., Ziervogel, K., Schnetzer, A., & Osburn, C. L. (2018). Formation of Chromophoric Dissolved Organic Matter by Bacterial Degradation of Phytoplankton-Derived Aggregates. Frontiers in Marine Science, 4. doi:10.3389/fmars.2017.00430. Table 3.
Methods

Shields, M. R., Bianchi, T. S., Osburn, C. L., Kinsey, J. D., Ziervogel, K., Schnetzer, A., & Corradino, G. (2019). Linking chromophoric organic matter transformation with biomarker indices in a marine phytoplankton growth and degradation experiment. Marine Chemistry, 214, 103665. doi:10.1016/j.marchem.2019.103665