Dataset: Trial B test of the dissolution method for estimates of the 15N2 atom% of incubations

Final no updates expectedDOI: 10.1575/1912/bco-dmo.778158.1Version 1 (2019-10-02)Dataset Type:experimental

Principal Investigator: Julie Granger (University of Connecticut)

Co-Principal Investigator: Annie Bourbonnais (University of Massachusetts Dartmouth)

Co-Principal Investigator: Samuel Wilson (University of Hawai'i)

BCO-DMO Data Manager: Mathew Biddle (Woods Hole Oceanographic Institution)

Project: EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean (EAGER NitFix)

Abstract

The “dissolution” method to measure N2 fixation rates with 15N2 gas tracer involves the preparation of 15N2-enriched water that is then added to each incubation bottle. Investigators typically measure the 15N2 atom% of the 15N2-enriched inoculum by MIMS, and extrapolate the 15N2 atom% in the incubations based on the inoculum value. Here, we demonstrate that such extrapolation yields inaccurate estimates of the 15N2 atom% of incubations. The latter should be measured directly.

Cite Dataset

XXX

Views

XX

Downloads

X

Citations

Trial b test of the dissolution method

Related Datasets

No Related Datasets

Related Publications

Results
White, A. E., Granger, J., Selden, C., Gradoville, M. R., Potts, L., Bourbonnais, A., Fulweiler, R. W., Knapp, A. N., Mohr, W., Moisander, P. H., Tobias, C. R., Caffin, M., Wilson, S. T., Benavides, M., Bonnet, S., Mulholland, M. R., & Chang, B. X. (2020). A critical review of the 15N2 tracer method to measure diazotrophic production in pelagic ecosystems. Limnology and Oceanography: Methods, 18(4), 129–147. Wiley. https://doi.org/10.1002/lom3.10353

Granger, J., Bourbonnais, A., Wilson, S. (2019) Trial B test of the dissolution method for estimates of the 15N2 atom% of incubations. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-10-02 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.778158.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

NSF Logo©2020 Biological and Chemical Oceanography Data Management Office.
Funded by the U.S. National Science Foundation