Arango et al. (2018) and Pyle et al. (2018) collectively describe 4 new species, three damselfish and a basslet. Specimens were collected with hand nets during deep dives using mixed-gas, closed-circuit rebreathers, and brought to the surface alive with the aid of a hypodermic needle to vent gas from the swim bladders. Methods for morphometric counts and measurements follow standard procedures as detailed in Pyle et al. (2016; Zookeys 641:165). DNA barcodes (COI) were produced with an ABI 3130XL genetic analyzer (Applied Biosystems, Foster City, California) at the Hawai'i Institute of Marine Biology EPSCoR Sequencing Facility.
Wilcox et al. (2018) and Tenggardjaja et al. (2018) are phylogeographic surveys. Fish fin clips were collected on shallow reefs with conventional SCUBA, using hand nets and pole spears. Tissue samples were preserved in salt-saturated DMSO buffer. Various mitochondrial and nuclear loci were sequenced for phylogeographic analyses. Collection data for Wilcox et al., including lat-long information, is provided in Table S2 (see Supplemental Files below). Locations for samples in Tenggardjaja et al. are limited to island sites (no lat-long available). See paper cited below for details.
In all cases the DNA sequences were aligned, edited and trimmed to a common length using Geneious Pro DNA analysis software (v.5.6.6 and v.6.2; Kearse et al. 2012; Bioinformatics 28: 1647–1649). Maximum Likelihood, Neighbor-Joining, and Maximum Parsimony tree-building methods were implemented using Mega v.5.2.2, and all sequences are available in GenBank. Where appropriate, population genetic parameters were calculated with ARLEQUIN 3.5 (Excoffier and Lischer 2010; Mol Ecol Resour 10:564–567).
