Dataset: Sediment trap chlorophyll a and phaeopigment flux from the "SalpPOOP" cruise on R/V Tangaroa during October and November 2018

Final no updates expectedDOI: 10.26008/1912/bco-dmo.813859.1Version 1 (2020-06-02)Dataset Type:Cruise Results

Principal Investigator: Michael R. Stukel (Florida State University)

Co-Principal Investigator: Moira Decima (New Zealand National Institute of Water and Atmospheric Research)

Co-Principal Investigator: Thomas Kelly (Florida State University)

Co-Principal Investigator: Scott Nodder (New Zealand National Institute of Water and Atmospheric Research)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Quantifying trophic roles and food web ecology of salp blooms of the Chatham Rise (Salp Food Web Ecology)


Abstract

Sediment trap chlorophyll a and phaeopigment flux from the "SalpPOOP" cruise on R/V Tangaroa during October and November 2018.

Data comes from VERTEX-style, surface-tethered, drifting sediment trap deployments. Particle interceptor tubes were deployed on cross-pieces with 16 tubes attached. Tubes were deployed with a dense formaldehyde brine created by adding NaCl and formaldehyde to filtered seawater. After recovery, overlying seawater was removed from each cruise by gentle suction. Tubes were then gravity filtered through a 200-micron nitex mesh filter, and the 200-micron filters were carefully analyzed under a stereomicroscope and all metazoan zooplankton "swimmers" were removed from the sample. Material remaining on the 200-micron filters (i.e., sinking material) was then imaged with a macrophotography rig and subsequently rinsed back into the original sample tube (i.e., re-combined with the <200-micron sinking material). Samples were then separated and filtered onto different types of filters for a suite of different analyses including: particulate organic carbon flux, particulate nitrogen flux, carbon and nitrogen isotopes, chlorophyll a and phaeopigment flux, microscopy, genetic analyses, and 234Th flux.

Triplicate 50-mL subsamples for Chlorophyll a and phaeopigments were filtered onto GF/F filters under low vacuum pressure. Samples were then placed in glass vials and frozen at -80C. They were later thawed out, and 7-mL of acetone was added. Samples were placed in a -20C freezer for 24 hours. Samples were then analyzed on a 10-AU fluorometer for Chl a and phaeopigments following Strickland and Parsons (1972).


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