Dataset: Cell abundance, growth rate, cellular P quotas, and alkaline phosphatase activity from a laboratory experiment examining the response of three species of marine phytoplankton grown under different phosphorus (P) conditions

Final no updates expectedDOI: 10.26008/1912/bco-dmo.820902.1Version 1 (2020-08-14)Dataset Type:experimental

Principal Investigator: LeAnn P. Whitney (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Michael W. Lomas (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Phosphonate Utilization by Eukaryotic Phytoplankton: Who, How, and Where? (Euk Phn Utilization)


Abstract

A laboratory experiment was carried out to characterize the growth and physiological response of three species of eukaryotic phytoplankton grown with inorganic phosphate and phosphonate as the sole sources of phosphorus (P). Data reported are cell abundance, growth rate, cellular P quotas, and alkaline phosphatase activity.

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Three species of marine phytoplankton – Micromonas commoda, Emiliania huxleyi, and Phaeodactylum tricornutum – were grown under four phosphorus (P) conditions. These include phosphate (Pi) replete and deplete conditions and the phosphonate conditions where cultures received either methylphosphonate (MPN) or 2-aminoethylphosphonate (2-AEPN) as the sole source of phosphorus at replete levels. Samples for cell abundance were collected throughout the experiment to monitor growth. In addition, particulate P, to calculate cellular P quota, as well as alkaline phosphatase activity was measured at a single time point when cells were in exponential growth.


Related Datasets

Different Version

Dataset: https://doi.org/10.5061/dryad.b765br4
Whitney, L. A. P., & Lomas, M. W. (2018). Data from: Phosphonate utilization by eukaryotic phytoplankton (Version 1) [Data set]. Dryad. https://doi.org/10.5061/DRYAD.B765BR4

Related Publications

Results

Whitney, L. P., & Lomas, M. W. (2018). Phosphonate utilization by eukaryotic phytoplankton. Limnology and Oceanography Letters, 4(1), 18–24. doi:10.1002/lol2.10100
Related Research

Lomas, M. W., Burke, A. L., Lomas, D. A., Bell, D. W., Shen, C., Dyhrman, S. T., & Ammerman, J. W. (2010). Sargasso Sea phosphorus biogeochemistry: an important role for dissolved organic phosphorus (DOP). Biogeosciences, 7(2), 695–710. doi:10.5194/bg-7-695-2010
Related Research

Whitney, L. P., & Lomas, M. W. (2016). Growth on ATP Elicits a P-Stress Response in the Picoeukaryote Micromonas pusilla. PLOS ONE, 11(5), e0155158. doi:10.1371/journal.pone.0155158