Dataset: Microbial cell counts in seawater samples collected on R/V Nerissa and R/V Yellowfin cruises at the San Pedro Ocean Time-series (SPOT) in 2017

Final no updates expectedDOI: 10.26008/1912/bco-dmo.827826.1Version 1 (2020-10-28)Dataset Type:Cruise Results

Principal Investigator: Sergio A. Sanudo-Wilhelmy (University of Southern California)

Contact: Laura Gómez-Consarnau (University of Southern California)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: The role of organic and metal cofactors on the biogenic synthesis of halogenated volatile hydrocarbons (Volatile_Hydrocarbons)


Abstract

This dataset contains microbial cell counts in seawater samples collected on R/V Nerissa and R/V Yellowfin cruises at the San Pedro Ocean Time-series (SPOT) in 2017. Samples for microbial cell counts were collected at six depths within the euphotic zone using Niskin bottles. Autotrophic picoplankton (Prochlorococcus, Synechococcus, and picoeukaryotes) and heterotrophic prokaryotes were enumerated by flow cytometry.

Samples for microbial cell counts were collected at six depths within the euphotic zone (5-250 m). Seawater was collected from each CTD depth using Niskin bottles, immediately fixed with formalin 2% (0.72% formaldehyde final concentration), and immediately frozen at -80C. Autotrophic picoplankton (Prochlorococcus, Synechococcus, and picoeukaryotes) and heterotrophic prokaryotes were enumerated by flow cytometry (Becton Dickinson FACSCalibur) (Gasol and del Giorgio, 2000).


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Methods

Gasol, J. M., & Del Giorgio, P. A. (2000). Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Scientia Marina, 64(2), 197–224. doi:10.3989/scimar.2000.64n2197