Dataset: Elemental composition of phytoplankton communities from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI.

Final no updates expectedDOI: 10.26008/1912/bco-dmo.848587.1Version 1 (2021-04-26)Dataset Type:Other Field Results

Principal Investigator, Contact: Stephanie I. Anderson (University of Rhode Island)

Co-Principal Investigator: Gayantonia Franzè (Institute of Marine Research (Bergen, Norway))

Co-Principal Investigator: David A. Hutchins (University of Southern California)

Co-Principal Investigator: Joshua D. Kling (University of California-Berkeley)

Co-Principal Investigator: Colin T. Kremer (University of California-Los Angeles)

Co-Principal Investigator: Elena Litchman (Michigan State University)

Co-Principal Investigator: Susanne Menden-Deuer (University of Rhode Island)

Co-Principal Investigator: Tatiana A. Rynearson (University of Rhode Island)

Co-Principal Investigator: Paul Wilburn (Michigan State University)

BCO-DMO Data Manager: Taylor Heyl (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Program: Dimensions of Biodiversity (Dimensions of Biodiversity)

Project: Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)


Abstract

This dataset reports the elemental composition of phytoplankton communities from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. These data were assessed in Anderson et al. The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community (in prep).

The elemental composition of phytoplankton communities was analyzed via multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. Water was incubated in triplicate at -0.5ºC, 2.6ºC, and 6ºC for 10 days. At each temperature, treatments included both nutrient amendments (N, P, Si addition) and controls (no macronutrients added).

At the onset and conclusion of the incubation for all treatments, and additionally at each dilution time point for 2.6 and 6ºC amended treatments, each biological incubation replicate was assessed for particulate organic carbon (POC) and nitrogen (PON), and biogenic silica (BSi) content. POC and PON were evaluated in triplicate by harvesting cells onto 25 millimeter (mm) GF/F filters which had been pre-combusted at 450ºC for 24 hours.  Filters were then analyzed on a Costech Elemental Combustion System (Costech Analytical Technologies Inc.). BSi content was assessed by filtering cells in triplicate onto 2 micrometer (µm) polycarbonate filters and analyzing them on a Barnstead Turner SP-830 spectrophotometer, following the methods of Strickland and Parsons (1972). Additionally, nutrient analyses of ammonium, phosphate, silicate, and nitrite/nitrate (total inorganic nitrogen) were evaluated using a Lachat Quikchem 8500 analyzer (Hach) at the University of Rhode Island Marine Science Research Facility.

For complete methodology, see “The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community” (Anderson et al, in prep).

Note: Due to operator error, one replicate of POC and PON had to be discarded from the 6ºC amended incubation.


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Anderson, S. I., Franze, G., Kling, J. D., Wilburn, P., Kremer, C. T., Menden-Deuer, S., Litchman, E., Hutchins, D. A., Rynearson, T. A. (2021) Microscopy cell counts from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-04-14 doi:10.26008/1912/bco-dmo.848977.1
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Franzè, G., Menden-Deuer, S., Anderson, S. I., Kling, J. D., Wilburn, P., Hutchins, D. A., Litchman, E., Rynearson, T. A. (2023) Herbivorous protist abundances under simultaneous manipulation of temperature and nutrients from the Long-term Plankton Time Series site in Narragansett Bay, RI in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-10 doi:10.26008/1912/bco-dmo.893414.1
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Franzè, G., Menden-Deuer, S., Anderson, S. I., Kling, J. D., Wilburn, P., Hutchins, D. A., Litchman, E., Rynearson, T. A. (2023) Temperature and nutrient dependent phytoplankton growth and herbivorous protist grazing rates from the Long-term Plankton Time Series site in Narragansett Bay, RI in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-12 doi:10.26008/1912/bco-dmo.893500.1

Related Publications

Results

Anderson, S. I., Franzè, G., Kling, J. D., Wilburn, P., Kremer, C. T., Menden‐Deuer, S., Litchman, E., Hutchins, D. A., & Rynearson, T. A. (2022). The interactive effects of temperature and nutrients on a spring phytoplankton community. Limnology and Oceanography, 67(3), 634–645. Portico. https://doi.org/10.1002/lno.12023
Methods

Strickland, J. D. H. and Parsons, T. R. (1972). A Practical Hand Book of Seawater Analysis. Fisheries Research Board of Canada Bulletin 157, 2nd Edition, 310 p.