Proportion of infected polyps at Days 31, 52 and 69 in newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.
Larvae were collected from Antillogorgia bipinnata colonies that were maintained in seawater tables at the Keys Marine Lab on Long Key, FL. Antillogorgia bipinnata colonies were initially collected from Tennessee Reef (N 24° 45.150' W 81° 45.275').
Breviolum antillogorgium cultures, representing five genotypes, were used to infect polyps reared from larvae collected from A. bipinnata. Three cultures had been grown at 26-degree C since isolation in 2016 (G1 [16-0590F], G2 [16-0875] and G3 [16-1631]) and two had been grown at 30-degree C since isolation in 2016 (G4 [16-0587] and G5 [16-0763]). Polyps from all treatments were reared at both 26 and 30 degree C in the lab under 12:12 light dark cycles. Containers with polyps were inoculated on Day 1 and Day 5. Starting on Day 32, containers were inoculated three times a week with water changes. Polyp location in the containers was mapped and infection status for each individual polyp was recorded every 3-4 days through visual observation as indicated by a light brown coloration in the polyp. At the end of the experiment (Day 69) infection status was based on cell counts.
Coffroth, M. A., terHorst, C. (2021) Proportion of infected polyps at Days 31, 52 and 69 in newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.855162.1 [access date]
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