Dataset: Particulate organic phosphorus data from TriCoLim incubation experiments done aboard R/V Atlantis AT39-05 in the Tropical Atlantic

Preliminary and in progressVersion 1 (2021-07-13)Dataset Type:Cruise Results

Principal Investigator, Contact: David A. Hutchins (University of Southern California)

Co-Principal Investigator: Feixue Fu (University of Southern California)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean (TriCoLim)


Abstract

Particulate organic phosphorus data from TriCoLim incubation experiments done aboard R/V Atlantis AT39-05 in the Tropical Atlantic

Shipboard incubation experiments were carried out at 15 stations in the tropical Atlantic with trace metal clean-collected bulk seawater to examine iron (Fe) and phosphorus (P) co-limitation of dinitrogen (N2) fixation (see TriCoLim Station coordinates metadata). Treatments were: Co-limited control (no additions, “Colimit”), + 2nM Fe (“Fe”), + 50 nM P (“P”) and Replete (+Fe+P, “Rep”). All incubations were carried out at ambient (“Lo”) temperature and ambient temperature +3C (“Hi”) for 2-4 days on deck before measuring 15N2 and 13CO2 fixation rates and particulate organic carbon (POC), nitrogen (PON) and phosphorus (POP). See related datasets.

POP measurements were conducted as follows: ~500 ml of bulk water were filtered onto pre-combusted GFF filters and rinsed two times with 2 ml each of 0.17M sodium sulfate (Na2SO4). Filters were placed in pre-combusted borosilicate vials capped a combusted aluminum foil cap and soaked in 2 ml of 0.017M magnesium sulfate (MgSO4) and left to dry in an oven at 60 deg C for 2-3 days.


Related Datasets

IsRelatedTo

Dataset: Colimitation Physiology
Relationship Description: Part of same experiment.
Hutchins, D. A., Fu, F. (2021) Physiology data from Crocosphaera iron and phosphorus colimitation culture experiment. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-20 http://lod.bco-dmo.org/id/dataset/856201

Related Publications

Results

Fu, F.-X., Zhang, Y., Bell, P. R. F., & Hutchins, D. A. (2005). PHOSPHATE UPTAKE AND GROWTH KINETICS OFTRICHODESMIUM(CYANOBACTERIA) ISOLATES FROM THE NORTH ATLANTIC OCEAN AND THE GREAT BARRIER REEF, AUSTRALIA. Journal of Phycology, 41(1), 62–73. doi:10.1111/j.1529-8817.2005.04063.x