Dataset: Diatom metabolites under P-limited and P-replete growth from laboratory cultures in July of 2013

Final no updates expectedVersion 1 (2021-08-27)Dataset Type:experimental

Principal Investigator: Elizabeth Kujawinski (Woods Hole Oceanographic Institution)

Technician: Krista Longnecker (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)


Program: Marine Microbiology Initiative (MMI)

Project: Identifying and quantifying new markers of microbially mediated nutrient flow in the ocean (Microbial metabolites)


Abstract

Diatom metabolites under P-limited and P-replete growth. Phosphorus limitation is pervasive in the oligotrophic surface ocean and marine microorganisms use different strategies to survive, and thrive, at these low nutrient levels. Eukaryotic algae such as diatoms are extremely sensitive to phosphorus limitation and recent transcriptomics work has suggested that multiple cellular processes are affected under these growth conditions. Metabolomics is the systematic study of intra- and extracellu...

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Raw Spectral Data Files are available in the MetaboLights database under study ID "MTBLS154" (https://www.ebi.ac.uk/metabolights/MTBLS154/).  Sample, assay, and metabolite information in ISAtab format are also available from that MetaboLights study.


Related Datasets

IsRelatedTo

Dataset: https://www.ebi.ac.uk/metabolights/MTBLS154/
Longnecker, K., Kujawinski, E. (2015) MTBLS154: Phosphorus availability regulates intracellular nucleotides in marine eukaryotic phytoplankton. MetaboLights Database. Release Date: 2015-01-27. Available at https://www.ebi.ac.uk/metabolights/MTBLS154/

Related Publications

Results

Kujawinski, E. B., Longnecker, K., Alexander, H., Dyhrman, S. T., Fiore, C. L., Haley, S. T., & Johnson, W. M. (2017). Phosphorus availability regulates intracellular nucleotides in marine eukaryotic phytoplankton. Limnology and Oceanography Letters, 2(4), 119–129. doi:10.1002/lol2.10043
Results

Longnecker, K., & Kujawinski, E. B. (2017). Mining mass spectrometry data: Using new computational tools to find novel organic compounds in complex environmental mixtures. Organic Geochemistry, 110, 92–99. doi:10.1016/j.orggeochem.2017.05.008
Methods

Dittmar, T., Koch, B., Hertkorn, N., & Kattner, G. (2008). A simple and efficient method for the solid-phase extraction of dissolved organic matter (SPE-DOM) from seawater. Limnology and Oceanography: Methods, 6(6), 230–235. doi:10.4319/lom.2008.6.230
Methods

Kuhl, C., Tautenhahn, R., Böttcher, C., Larson, T. R., & Neumann, S. (2011). CAMERA: An Integrated Strategy for Compound Spectra Extraction and Annotation of Liquid Chromatography/Mass Spectrometry Data Sets. Analytical Chemistry, 84(1), 283–289. doi:10.1021/ac202450g
Methods

Rabinowitz, J. D., & Kimball, E. (2007). Acidic Acetonitrile for Cellular Metabolome Extraction fromEscherichia coli. Analytical Chemistry, 79(16), 6167–6173. doi:10.1021/ac070470c