Dataset: T. rotula microbiome global sample

Single cells and chains of T. rotula were subjected to three serial washes in separate aliquots of 200 μl sterile seawater, with <2 μl seawater carried between washes. Individual cells or chains were then cultured with associated bacteria in 1ml f/20 in sterile polystyrene plates at ~in situ temperature and 90-120 μmol photons m⁻² s⁻¹ on a 12:12-h light:dark cycle. Isolates were grown for two weeks, or until the phytoplankton concentration reached ~1,000 cells mL⁻¹, whichever occurred sooner. Single cell isolates were filtered onto 0.2-μm filters (Millipore) and stored at -80 °C until DNA extraction. DNA was extracted from T. rotula isolates and bacterial co-cultures following Whittaker and Rynearson (2017).