Dataset: T. rotula microbiome global sample

Final no updates expectedDOI: 10.26008/1912/bco-dmo.860347.1Version 1 (2021-09-13)Dataset Type:Other Field Results

Principal Investigator: Tatiana A. Rynearson (University of Rhode Island)

Contact: Olivia Ahern (University of Rhode Island)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Connecting local, regional and global scales of gene flow in planktonic marine diatoms (Diatom Gene Flow)


Abstract

This dataset includes NCBI identifiers and descriptions of T. rotula microbiome global samples.

Single cells and chains of T. rotula were subjected to three serial washes in separate aliquots of 200 μl sterile seawater, with <2 μl seawater carried between washes. Individual cells or chains were then cultured with associated bacteria in 1ml f/20 in sterile polystyrene plates at ~in situ temperature and 90-120 μmol photons m⁻² s⁻¹ on a 12:12-h light:dark cycle. Isolates were grown for two weeks, or until the phytoplankton concentration reached ~1,000 cells mL⁻¹, whichever occurred sooner. Single cell isolates were filtered onto 0.2-μm filters (Millipore) and stored at -80 °C until DNA extraction. DNA was extracted from T. rotula isolates and bacterial co-cultures following Whittaker and Rynearson (2017).


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Results

Ahern, O. M., Whittaker, K. A., Williams, T. C., Hunt, D. E., & Rynearson, T. A. (2021). Host genotype structures the microbiome of a globally dispersed marine phytoplankton. Proceedings of the National Academy of Sciences, 118(48). https://doi.org/10.1073/pnas.2105207118
Methods

Whittaker, K. A., & Rynearson, T. A. (2017). Evidence for environmental and ecological selection in a microbe with no geographic limits to gene flow. Proceedings of the National Academy of Sciences, 114(10), 2651–2656. doi:10.1073/pnas.1612346114