Dataset: Bioavailability factor of polyphosphate, nucleotides, and methyl phosphonate in bioassay experiments with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring of 2019

Release Date:2022-07-26Data not availableVersion 1 (2021-11-02)Dataset Type:Cruise ResultsDataset Type:experimental

Principal Investigator: Solange Duhamel (Columbia University)

Co-Principal Investigator: Julia Diaz (University of Georgia)

Contact: Kahina Djaoudi (University of Arizona)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle (P-hydrolase)


Abstract

Bioavailability factor of polyphosphate, nucleotides (ATM and AMP) and methyl phosphonate (Mepn) in bioassay experiments with seawater collected during R/V Savannah cruise SAV-19-02 from March to April of 2019 in the Northwestern Atlantic from the surface to 50 m depth.

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Sampling and analytical procedures:

Bioassay experiments were conducted at station 1 and stations 3. At each station, inorganic and organic phosphate amendments were performed on seawater with and without nitrogen enrichment  (NH4Cl, NaNO3). Bioassay Experiments consisted in incubating, over an incubation period of 48h, surface seawater (5m) with inorganic or organic phosphate compounds (20 µM; final concentration of P) including, polyphosphate (polyp), inorganic phosphate (Pi), nucleotides (ATP or AMP) and methylphosphonate (Mepn). In each incubation experiment, a control treatment (surface seawater without amendment) was included.

In bioassay experiments, the bioavailability factor (BF) was determined at T0 for polyphosphate, nucleotides (ATP or AMP) and methylphosphonate (Mepn). BF is calculated as follows: BF=TE-TN/ TP-TN (Björkman and Karl, 1994), where TE reflects PO43- turnover time in the DOP amended treatment, TN is the PO43- turnover time in the control (no additions) and TP is the PO43- turnover time in the treatment amended with Pi. BF ranges from 0 for an unavailable substrate, to a value of 1 for a DOP model substrate having a bioavailability equal to that of +Pi.

Instrument: Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter. 
Location:  Northwestern Atlantic surface waters. Depth: surface-50 m.


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Methods

Björkman, K., & Karl, D. M. (1994). Bioavailability of inorganic and organic phosphorus compounds to natural assemblages of microorganisms in Hawaiian coastal waters. Marine Ecology Progress Series, 111(3), 265–273. http://www.jstor.org/stable/24849565