Bioavailability factor of polyphosphate, nucleotides (ATP and AMP) and phosphonate in seawater collected during R/V Savannah cruise SAV-19-02 from March to April of 2019 in the Northwestern Atlantic from the surface to 50 m depth.
Sampling and analytical procedures:
Seawater samples for bioavailability factor determinations were collected from Niskin bottles (12 L), at station 1 (two measurements) and 3 (one measurement) along a transect from coastal Georgia to offshore waters. The bioavailability factor was calculated based on changes in PO43- turnover time. The bioavailability factor (BF) is calculated as follows: BF=TE-TN/ TP-TN (Björkman and Karl, 1994), where TE reflects PO43- turnover time in the DOP amended treatment, TN the PO43- turnover time in the control (no additions) and TP the PO43- turnover time in the treatment amended with Pi. In this study, treatments were amended with polyphosphate (Polyp), adenosine triphosphate (ATP), adenosine monophosphate (AMP) or methyl phosphonate (Mepn).
BF ranges from 0 for an unavailable substrate, to a value of 1 for a DOP model substrate having a bioavailability equal to that of +Pi.
Instruments: Sampling was performed using Niskin bottles (12 L) mounted on a rosette. Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter.
Location: Northwestern Atlantic surface waters. Depth: surface-50 m.
Problem report: BF in station 1 (first determination) were determined in duplicates instead of triplicate in station 1 (second determination) and station 3. Therefore, gaps are filled with NaN in the .mat files.
Duhamel, S., Diaz, J. (2021) Bioavailability factor of polyphosphate, nucleotides (ATP and AMP) and phosphonate in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-02 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/864280 [access date]
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