©2020 Biological and Chemical Oceanography Data Management Office.Volume-dependent N2O blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted.
Methodology:
Sampling and analytical procedures:
Effects of sample volume on the size of bacterial blanks
Following sparging with N2 gas, bacterial concentrates (2 mL) in 20 mL vials were injected with incremental volumes of DIW or NO3--deplete surface Sargasso seawater. Solutions included air-equilibrated DIW (for ≥ 1 day) and helium-sparged DIW (for 30 minutes), as well as air-equilibrated vs. helium-sparged seawater. N2O yields were estimated from peak areas recovered by mass spectrometric analysis, calibrated with standard additions.
Zhou, M., Granger, J., Chang, B. X. (2022) Volume effects on blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted from 2017-2020 (Biological Nitrogen Isotope Fractionation project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.865089.1 [access date]
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