Dataset: GenBank accession links of the invertebrates collected from Autonomous Reef Monitoring Structures (ARMS) from shallow fringing reefs near Dobu and Upa Upasina, Milne Bay Province, Papua New Guinea in 2014

Sampling Devices
This study was conducted in Milne Bay Province, Papua New Guinea at two shallow-water fringing reef localities near Dobu and Upa Upasina (9° 49.45’ S, 150° 49.06’ E) at a depth of 3 meters where almost pure CO2 gas seeps through the seafloor and into the water, changing the water carbonate chemistry and creating sharp gradients in seawater carbonate chemistry parameters within a short distance. Autonomous Reef Monitoring Structures (ARMS) were deployed along the fringing coral reefs at a depth of 4-5 meters at three pH conditions and recovered after approximately 2 years of deployment and all motile organisms greater than 2 millimeters were removed from the structures, photographed, subsampled for genetic analysis, and preserved in 95% ethanol.

Genetic Barcoding
DNA was extracted from tissue subsamples using a standard proteinase-k digestion followed by phenol-chloroform extraction on an AutoGenprep 965. The standard Cytochrome Oxidase Subunit I (COI) barcoding fragment (658 bp) was amplified using the jgLCO1490/jgHCO2198 primer pair. Automated sequencing was performed in both directions directly on purified PCR products using Applied Biosystems BigDye terminator V3.1 Sequence reactions were purified using Millipore 96-well plates loaded with Sephadex G-50 and run on an ABI 3130xl genetic analyzer.