Dataset: Microsatellite and chloroplast 23S genotypes of Breviolum sp. symbionts within Orbicella faveolata adults from reefs in the Florida Keys from 2009-2011 (SymBioSys project)

Sampling and Analytical Methodology

Tissue samples were collected from the top, middle and bottom portions of adult Orbicella faveolata colonies using the syringe method as described in Correa et al. (2009) and DNA extracted following Coffroth et al. (1992). 

Genotypes of the algal symbionts within the genus Breviolum were characterized using three polymorphic microsatellite loci, B7Sym34, B7Sym36 and CA6.38, which were adapted for use with O. faveolata (Thornhill et al. 2009).

Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003).

Locations in the Florida Keys (Table of reef abbreviations)

Reef
Abbreviation

Alligator
AR

Coral Garden
CG

Cheeca Rocks
CR

East Turtle
ET

Grecian Rocks
GR

Looe Key
LK

Sand Island
SI

Tennessee
TR