Dataset: Breviolum symbiont CP23S genotypes in Orbicella faveolata recruits from 2009-2011 (SymBioSys project)

Sampling and Analytical Methodology: 

Egg-sperm bundles were collected from adult colonies of O. faveolata following the techniques described by Miller (2014).  Larvae were reared at a shore-based lab and settled onto terracotta tiles.  Once the larvae had metamorphosed and attached to the tiles, they were outplanted onto the reef by attachment vertically to a PVC rack approximately 0.2 m above the substrate to allow uptake of algal symbionts. Tiles with recruits were sampled one to three months after outplanting, recruits removed from the tile and preserved individually in 95% ethanol for subsequent molecular analysis. DNA extraction followed Coffroth et al. (1992).    Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003).

Locations in the Florida Keys with Abbreviations

• Alligator (AR)
• Coral Garden (CG)
• Cheeca Rocks (CR)
• East Turtle (ET)
• Grecian Rocks (GR)
• Looe Key (LK)
• Sand Island (SI)
• Tennessee (TR)