Dataset: Environmental data, nutrients, and leucine and thymidine bacterial production from samples collected by CTD during cruises in the San Pedro Channel on R/V Yellowfin from 2005 to 2018

Monthly San Pedro Ocean Time-series (SPOT) cruises on R/V Yellowfin were conducted in the San Pedro Channel, off the coast of Los Angeles, California, USA (33 N, 118 W). Variables including temperature, oxygen, and fluorescence were recorded by a Seabird Scientific SBE25plus Sealogger CTD at the time of sample collection. Nutrient variables including nitrite, nitrate, and phosphate were analyzed by MSI Analytical Lab at the University of California Santa Barbara. Satellite sea surface temperature, chlorophyll-a concentration, and surface productivity estimates were downloaded from the NOAA Coastwatch browser website. Leucine and thymidine bacterial production assays were performed by incubating seawater with H3-tagged leucine or thymidine for one hour, killing with trichloroacetic acid, filtered onto a 0.45 µm MCE filter (EMD Millipore HAWP02500). Activity on the filters is measured using a liquid scintillation counter (Beckman Coulter LS6500), and then the rate of uptake is used to calculate bacterial cells per milliliter of each sample.