Dataset: Seawater concentration data from an ocean acidification exposure experiment on adult Eastern oysters from Plum Island Sound in 2017

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.888887.1Version 1 (2023-02-02)Dataset Type:Other Field Results

Principal Investigator: Katie Lotterhos (Northeastern University)

Co-Principal Investigator, Contact: Justin B. Ries (Northeastern University)

Student: Elise McNally (Northeastern University)

BCO-DMO Data Manager: Taylor Heyl (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Does ocean acidification induce a methylation response that affects the fitness of the next generation in oysters? (Epigenetics to Ocean)


Abstract

Trace, minor, and major element data from adult Eastern oyster ocean acidification exposure experiments were conducted at the Ries Lab at the Northeastern University Marine Science Center on samples from Plum Island Sound in 2017. This dataset represents the phenotypic and molecular responses in the extrapallial fluid in the adult eastern oyster (Crassostrea virginica) exposed to experimental ocean acidification (OA) over 80 days.

In this study, we examined the phenotypic and molecular responses in the extrapallial fluid in the adult eastern oyster (Crassostrea virginica) exposed to experimental ocean acidification (OA) over 80 days. The collection and culturing of C. virginica specimens are detailed in Downey-Wall, A.M., L.P. Cameron, B.M. Ford, E.M. McNally, Y.R. Venkataraman, S.B. Roberts, J.B. Ries, and K.E. Lotterhos. 2020. Ocean acidification induces subtle shifts in gene expression and DNA methylation in the mantle tissue of the Eastern oyster (Crassostrea virginica). Frontiers in Marine Science doi: 10.3389/fmars.2020.566419.

Extrapallial fluid (EPF) was extracted as described in Downey-Wall et al. (2020). Extrapallial fluid was extracted by inserting a sterile 5 milliliter (mL) syringe with a flexible 18-gauge polypropylene tip into the EPF cavity through the luer-lock port. The EPF was stored in 2 mL polypropylene microcentrifuge tubes with screw caps (Fisherbrand Catalog No. 02-682-558) and refrigerated at 6 degrees celsius until further analysis.

Since all tanks received water from the same header source, seawater from a subset of six tanks (2 treatment-1) was sampled for elemental analysis. Seawater samples were collected in 50-milliliter (mL) polypropylene centrifuge tubes outside of an oyster sampling timepoint near the halfway point of the experimental exposure (day 63).

Elemental analysis
Extrapallial fluid and seawater were analyzed for trace and minor elements by inductively coupled plasma mass spectrometry (ICPMS). Liquid samples (i.e., EPF, seawater) were diluted to less than 0.05 percent total dissolved solid content with ultra-pure deionized water in 15 mL polypropylene centrifuge tubes and acidified with ultra-pure nitric acid (Fisher TraceMetal Grade Nitric Acid UN2031).

Extrapallial fluid and seawater was analyzed for a suite of 57 elements (including Ca) by ActLabs, Ontario, Canada. Liquid samples were analyzed using the ActLabs ICPMS method.

 

 


Related Datasets

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Dataset: Ocean Acidification Experiment: Phenotypic responses of Eastern oyster
Downey-Wall, A., Lotterhos, K., Ries, J. B., Cameron, L. (2023) Phenotypic responses of Eastern oyster in response to variable length OA exposure conducted in summer 2017 with oysters sampled in Plum Island. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-20 http://lod.bco-dmo.org/id/dataset/887553
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Dataset: Molar Ratios
Lotterhos, K., Ries, J. B. (2023) Molar Ratios from an adult Eastern oyster ocean acidification exposure experiment at the Northeastern University Marine Science Center in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-02 doi:10.26008/1912/bco-dmo.888911.1
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Dataset: Shell Concentrations
Lotterhos, K., Ries, J. B. (2023) Shell Concentrations from an adult Eastern oyster ocean acidification exposure experiment on adult Eastern oysters from Plum Island Sound in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-02 doi:10.26008/1912/bco-dmo.888902.1

Related Publications

IsRelatedTo

Downey-Wall, A. M., Cameron, L. P., Ford, B. M., McNally, E. M., Venkataraman, Y. R., Roberts, S. B., Ries, J. B., & Lotterhos, K. E. (2020). Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica). Frontiers in Marine Science, 7. https://doi.org/10.3389/fmars.2020.566419