Dataset: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)

Name: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)
Published: 2023-04-18
Keywords: RB-TnSeq, gene fitness, transporters, metabolism, arrayed mutant library, genetic screen, oceans

Final no updates expectedDOI: 10.26008/1912/bco-dmo.894179.1Version 1 (2023-04-18)Dataset Type:experimental

Principal Investigator: Mary Ann Moran (University of Georgia)

Co-Principal Investigator: Christopher R. Reisch (University of Florida)

Scientist: Catalina Mejia (University of Florida)

Scientist: Lidimarie Trujillo Rodriguez (University of Florida)

Student, Contact: William F. Schroer (University of Georgia)

Data Manager: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Program: Center for Chemical Currencies of a Microbial Planet (C-CoMP)

Project: Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites (C-CoMP Marine Bacterial Transporters)

Abstract

High resolution growth screens were used to confirm the phenotype of Ruegeria pomeroyi DSS-3 transporter knockout mutants. Mutants which had demonstrated growth defects on a given substrate as sole carbon source during an initial growth screen were selected and used here. Each mutant was grown on the substrate(s) of interest along side a wildtype analog (pooled-TnSeq library). Growth curves were generated by reading the optical density at 600 nm hourly. The annotations of a transporter's cognate...

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Each column heading provides the treatment condition and replicate. The first 4 letters are substrate abbreviation (provided below) followed by the bacterial identifier. The bacterial identity is either "WT" indicating the wildtype analog (pooled-RBTnSeq library) or four numbers indicating the locus tag of the disrupted gene (e.g. "1234" represents a mutant of "SPO1234"). Finally, the underscore and lowercase letter ("_a", "_b", "_c", "_d") indicates the biological replicate.

Values are optical density at 600 nm.

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Results

Schroer, W. F., Kepner, H. E., Uchimiya, M., Mejia, C., Rodriguez, L. T., Reisch, C. R., & Moran, M. A. (2023). Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites. https://doi.org/10.1101/2023.01.19.524783

Results

Schroer, W. F., Kepner, H. E., Uchimiya, M., Mejia, C., Rodriguez, L. T., Reisch, C. R., & Moran, M. A. (2023). Functional annotation and importance of marine bacterial transporters of plankton exometabolites. ISME Communications, 3(1). https://doi.org/10.1038/s43705-023-00244-6

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Dataset: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)

Final no updates expectedDOI: 10.26008/1912/bco-dmo.894179.1Version 1 (2023-04-18)Dataset Type:experimental

Principal Investigator: Mary Ann Moran (University of Georgia)

Co-Principal Investigator: Christopher R. Reisch (University of Florida)

Scientist: Catalina Mejia (University of Florida)

Scientist: Lidimarie Trujillo Rodriguez (University of Florida)

Student, Contact: William F. Schroer (University of Georgia)

Data Manager: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Program: Center for Chemical Currencies of a Microbial Planet (C-CoMP)

Project: Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites (C-CoMP Marine Bacterial Transporters)

Abstract

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Values are optical density at 600 nm.

Related Datasets

No Related Datasets

Related Publications

Results

Dataset: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)

Principal Investigator: Mary Ann Moran (University of Georgia)

Co-Principal Investigator: Christopher R. Reisch (University of Florida)

Scientist: Catalina Mejia (University of Florida)

Scientist: Lidimarie Trujillo Rodriguez (University of Florida)

Student, Contact: William F. Schroer (University of Georgia)

Data Manager: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Program: Center for Chemical Currencies of a Microbial Planet (C-CoMP)

Project: Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites (C-CoMP Marine Bacterial Transporters)

Abstract

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XX

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Related Datasets

Related Publications

Dataset: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)

Principal Investigator: Mary Ann Moran (University of Georgia)

Co-Principal Investigator: Christopher R. Reisch (University of Florida)

Scientist: Catalina Mejia (University of Florida)

Scientist: Lidimarie Trujillo Rodriguez (University of Florida)

Student, Contact: William F. Schroer (University of Georgia)

Data Manager: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Program: Center for Chemical Currencies of a Microbial Planet (C-CoMP)

Project: Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites (C-CoMP Marine Bacterial Transporters)

Abstract

XXX

XX

X

Files

Related Datasets

Related Publications

Dataset: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)

Principal Investigator: Mary Ann Moran (University of Georgia)

Co-Principal Investigator: Christopher R. Reisch (University of Florida)

Scientist: Catalina Mejia (University of Florida)

Scientist: Lidimarie Trujillo Rodriguez (University of Florida)

Student, Contact: William F. Schroer (University of Georgia)

Data Manager: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Program: Center for Chemical Currencies of a Microbial Planet (C-CoMP)

Project: Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites (C-CoMP Marine Bacterial Transporters)

Abstract

Metadata

XXX

XX

X

Files

Related Datasets

Related Publications

Dataset: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)

Principal Investigator: Mary Ann Moran (University of Georgia)

Co-Principal Investigator: Christopher R. Reisch (University of Florida)

Scientist: Catalina Mejia (University of Florida)

Scientist: Lidimarie Trujillo Rodriguez (University of Florida)

Student, Contact: William F. Schroer (University of Georgia)

Data Manager: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Program: Center for Chemical Currencies of a Microbial Planet (C-CoMP)

Project: Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites (C-CoMP Marine Bacterial Transporters)

Abstract

Metadata

XXX

XX

X

Files

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