Dataset: Dissolved trace metal (Mn, Fe, Co, Ni, Cu, Zn, Cd, Pb) and labile particulate elemental (P, V, Mn, Fe, Co, Ni, Cu, Zn, Cd, Pb) concentrations from shipboard incubation experiments conducted on the 2018 EXPORTS cruise (RR1813) near Ocean Station PAPA

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.896884.1Version 1 (2023-06-06)Dataset Type:Cruise Results

Principal Investigator, Contact: Kristen Nicolle Buck (University of South Florida)

Co-Principal Investigator: Mark A. Brzezinski (University of California-Santa Barbara)

Co-Principal Investigator: Bethany D. Jenkins (University of Rhode Island)

Student: Shannon M. Burns (University of South Florida)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Program: EXport Processes in the Ocean from Remote Sensing (EXPORTS)

Project: Collaborative Research: Diatoms, Food Webs and Carbon Export - Leveraging NASA EXPORTS to Test the Role of Diatom Physiology in the Biological Carbon Pump (Diatoms and carbon export)


Abstract

This dataset includes concentrations of dissolved (<0.4 micrometers (µm)) and labile particulate (0.4-5 µm and >5 µm) phosphorus (P), vanadium (V), manganese (Mn), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) in shipboard incubation samples collected during the EXports Processes in the Oceans from RemoTe Sensing (EXPORTS) North Pacific (NP) cruise RR1813 on the R/V Roger Revelle near Ocean Station PAPA (Station P).

Seawater for six shipboard incubation experiments was collected using a surface towfish (Mellett and Buck 2020) at ~2 meters (m) depth on the R/V Roger Revelle between 15 August 2018 and 29 August 2018. Water was prefiltered through a 150-µm mesh to remove large grazers. For incubations (Inc) 1-2 and 4-5, water was homogenized into 20-liter (L) carboys, and three carboys were amended for each treatment indicated in Table 1 (see Supplemental Files), yielding triplicates for each treatment and timepoint sampled. Each carboy was sampled once at the indicated timepoints. Control carboys sampled at the beginning and end of each incubation were unique to the timepoint (i.e., no carboy was resampled over time). For Incs 3 and 6, water was first homogenized into two 20-L carboys, from which 4-L bottles were filled and amended with the treatments indicated in Table 1. Three 4-L bottles were amended for each treatment, and each 4-L bottle was sampled once at the indicated timepoints, yielding triplicates.

Incubations 1 and 4 were carried out for 24 hours, Inc 2 and 3 for 6 days, and Inc 5 and 6 for 8 days (Table 1). The nutrient amendments for each experiment were selected to induce nutrient stress. For Inc 2 and Inc 5, we refer to the +20 μM nitrate +1.25 μM phosphate +20 μM silicic acid treatment as "AllButFe" and the +20 μM nitrate +1.25 μM phosphate +5 nM ⁵⁷FeCl₃ treatment as "AllButSi".

Dissolved (<0.4 μm) trace metal samples were collected in acid-cleaned 125-milliliter (mL) low-density polyethylene (LDPE, Nalgene) bottles, and acidified to 0.024 M hydrochloric acid (HCl, Fisher Optima). Dissolved trace metal (Fe, Cu, Mn, Co, Ni, Cu, Zn, Cd, Pb) concentrations were determined by high-resolution inductively coupled plasma mass spectrometry (HR-ICP-MS) at the University of South Florida (USF) on an Element XR (Hollister et al. 2020; Burns et al. 2023).

Leachable particulate trace metals were collected on 0.4-μm and 5-μm polycarbonate track etch (PCTE) filters, transferred into 1.5-mL high-density polyethylene (HDPE) snap-cap vials, and frozen at -20 degrees Celsius until processed. Filters were leached in a heated 25% acetic acid leach with a reducing step, "Berger Leach", at National High Magnetic Field Laboratory (NHMFL) to extract the leachable particulate trace metals (Berger et al. 2008). Leachable particulate elemental (P, V, Fe, Cu, Mn, Co, Ni, Cu, Zn, Cd, Pb) concentrations were determined by high-resolution inductively coupled plasma mass spectrometry (HR-ICP-MS) on an Element 2 with the assistance of Dr. Peter Morton of Florida State University (FSU).

Sample analyses for dissolved trace metals and leachable particulate trace metals were performed by Shannon Burns (USF). Detection limits and quality control results are presented in Table 2 (see Supplemental Files).


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Methods

Berger, C. J. M., Lippiatt, S. M., Lawrence, M. G., & Bruland, K. W. (2008). Application of a chemical leach technique for estimating labile particulate aluminum, iron, and manganese in the Columbia River plume and coastal waters off Oregon and Washington. Journal of Geophysical Research, 113. doi:10.1029/2007jc004703
Methods

Burns, S. M., Bundy, R. M., Abbott, W., Abdala, Z., Sterling, A. R., Chappell, P. D., Jenkins, B. D., & Buck, K. N. (2023). Interactions of bioactive trace metals in shipboard Southern Ocean incubation experiments. Limnology and Oceanography, 68(3), 525–543. Portico. https://doi.org/10.1002/lno.12290
Methods

Hollister, A. P., Kerr, M., Malki, K., Muhlbach, E., Robert, M., Tilney, C. L., Hubbard, K.A., & Buck, K. N. (2020). Regeneration of macronutrients and trace metals during phytoplankton decay: An experimental study. Limnology and Oceanography. doi:10.1002/lno.11429
Methods

Mellett, T., & Buck, K. N. (2020). Spatial and temporal variability of trace metals (Fe, Cu, Mn, Zn, Co, Ni, Cd, Pb), iron and copper speciation, and electroactive Fe-binding humic substances in surface waters of the eastern Gulf of Mexico. Marine Chemistry, 227: 103891. doi:10.1016/j.marchem.2020.103891