Dataset: High throughput growth screening of the marine bacterium Ruegeria pomeroyi DSS-3 knockout mutants on 70 environmentally relevant marine substrates done in 2021.

The data were generated from laboratory experiments performed at the University of Florida, Gainesville, Florida. 

Mutant cultures were pre-grown overnight in ½ YTSS medium with 50 mg ml-1 kanamycin. Screens were performed in L1 minimal medium (Guillard and Hargraves 1993) modified to a salinity of 20 ppt and amended with ammonium (3 mM), kanamycin (50 mg ml-1), and phosphorus as PO43- at 36 mM. Overnight cultures of individual mutants (2 ml) were inoculated into 198 ml of modified L1 with a single substrate as the sole carbon source at 8 mM carbon. Plates were incubated at 25oC with shaking, and optical density (OD600) was read at intervals of 6-24 h until cultures entered stationary phase at ~24-72 h (SpectraMax M3, Molecular Devices, San Jose, CA). Mutants exhibiting phenotypes in the initial screen were moved to the targeted screen.