File(s) | Type | Description | Action |
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922101_v1_hrr_18s_blast_results.csv (117.16 MB) | Comma Separated Values (.csv) | Primary data file for dataset ID 922101, version 1 | Download |
This dataset includes results of 18S sequencing of full-length 18S rDNA for metabarcoding samples collected from surface depth at stations 06, 11, 16, 21, SS, and GI during R/V Pt. Sur cruise PS18-09 Leg 1 using Nanopore minION.
On September 23, 2017, on HRR cruise Leg 1 (R/V Point Sur PS18-09), samples were collected at 6 stations (S06, S11, S16, S21, SS, and GI) from the surface depth bottle in a CTD rosette. Triplicate 500-1000 milliliter (mL) samples were filtered and immediately fixed in RNALater. Triplicate samples from each station/depth were extracted with AllPrep DNA/RNA MiniKit (Qiagen, USA) following the manufacturer's instructions. DNA concentration and quality were evaluated using a Nanodrop spectrophotometer (Thermo Fisher Scientific Inc, USA). All the samples extracted for DNA were normalized to 5 nanograms per microliter (ng/µL) concentration for the amplicon library construction.
The full-length 18S rDNA region was amplified using 18S primers (Medlin et al. 1988; Edgar & Theriot 2004). Library construction and amplicon sequencing were performed at the Campbell Lab, Texas A&M University using Oxford Nanopore minION. The output sequencing results as fasta files were deposited in NCBI's GenBank under the project number PRJNA592369.
Campbell, L., Henrichs, D. W., Gaonkar, C. (2024) Results of 18S sequencing of full-length 18S rDNA for metabarcoding samples collected during R/V Point Sur cruise PS18-09 in the Western Gulf of Mexico in September 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.922101.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.