This dataset includes data from experiment 2, residence time and irradiance, described in the manuscript: Optimizing marine macrophyte capacity to locally ameliorate ocean acidification under variable light and flow regimes: Insights from an experimental approach (2023). An experiment examining the effects of water residence time upon S. latissima capacity to ameliorate seawater acidity under ambient and simulated future scenarios of climate change in a gradient of irradiance was conducted in...
Show moreMethodology summary: The experiment in aquaria measured changes in dissolved oxygen, pH, and total alkalinity, and derived resultant changes to dissolved inorganic carbon (DIC) and calcium carbonate saturation state (Ω).
Values of temperature, salinity and oxygen were measured using a multiparameter meter (HQ40D, Hach Lange). Discrete water samples were acquired from each tank for analysis of pH and total alkalinity (AT) that were run the same day of the experiment. Seawater pH of the discrete water samples was determined on the total scale as per Dickson et al. 2007 with a high-precision spectrophotometer (Agilent Cary 8454 with PCB 1500 Water Peliter System) using unpurified m-cresol purple dye (Alfa Aesar A180025-06). Water samples were kept in a temperature-controlled water bath at 20°C before analysis, and during analysis using a thermostatic cell holder, to minimize temperature-induced errors in absorbance measurements. The spectrophotometer was validated by analyzing TRIS buffer revealing that the system was accurate to within 0.005 pH units. Seawater AT was measured using a CONTROS HydroFIA TA® instrument (4H-Jena GmbH, Jena Germany), which performed a single-point titration of seawater with 0.1N HCl, using bromocresol green as the indicator for spectrophotometric pH detection. HCl titrant was calibrated with Certified Reference Material (Batch 172) from A. Dickson’s laboratory. Instrumental precision was within 5 µmol kg -1.
See detailed methodology in Ricart AM, Honisch B, Fachon E, Hunt CW, Salisbury J, Arnold SN, Price NN. Optimizing marine macrophyte capacity to locally ameliorate ocean acidification under variable light and flow regimes: Insights from an experimental approach. PLoS One. 2023 Oct 11;18(10):e0288548. doi: 10.1371/journal.pone.0288548. PMID: 37819926; PMCID: PMC10566731.
Ricart, A. M., Price, N. N., Honisch, B. (2024) Macrophytes' amelioration of seawater acidity: Residence time and irradiance in the Gulf of Maine on 2018-11-01. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-05-21 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/922819 [access date]
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