Dataset: Hatching count and survival statistics of Atlantic silverside (Menida menidia) larvae reared under ambient or hypoxia/acidification treatments during cross-generational laboratory experiments in 2022

ValidatedFinal no updates expectedVersion 1 (2024-04-16)Dataset Type:experimental

Principal Investigator: Christopher S. Murray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)


Project: OCE-PRF Detecting signatures of multigenerational plasticity in a marine forage fish (HypOA Cross-generational Plasticity)


Abstract

This dataset contains daily counts of hatching Atlantic silverside (Menidia menidia) larvae during cross-generational laboratory experiments testing how parental exposure to a combined hypoxia and acidification treatment (hereafter HypOA) affected offspring responses and HypOA tolerance in an important coastal forage fish species. Embryo and larval survival statistics by replicate are provided as a supplemental file in this dataset.

Study description:
*see "Related Datasets" section for access to related datasets from this experiment

These data were generated during a cross-generational laboratory experiment testing how parental exposure to a combined hypoxia and acidification treatment affected offspring responses and HypOA tolerance in an important coastal forage fish species, Atlantic silverside Menidia menidia. The experiment was conducted during June and July 2022 at Woods Hole Oceanographic Institution’s Environmental Systems Laboratory.   Wild adult Atlantic silversides were conditioned to two treatment levels: control (100% dissolved oxygen [DO] / 650 µatm pCO2) or HypOA conditions (40% DO / 2300 µatm pCO2) for 10 days. After artificial fertilization, their offspring were reared in a factorial experimental design to test how parental conditioning to the stressors affected the survival, development, and growth of embryos and larvae when reared under control or HypOA conditions. The larvae were sampled for morphometric measurements at hatch (9 or 10 days post-fertilization) and after 10 days of larval rearing (19 days post-fertilization). We evaluated survival to hatch (fertilization to 10 days post-fertilization), during larval development (hatch to 10 days post hatch), and over the entire rearing interval (fertilization to 19 days post-fertilization). We found that direct offspring exposure to HypOA lowered survival and growth; however, embryos fertilized by HypOA conditioned parents showed better survival under HypOA conditions. By contrast, we found that larvae from HypOA-conditioned parents exhibited, on average, slower post-hatch growth and slightly lower larval survival in offspring groups reared under both treatment conditions. The data demonstrate that parental conditioning does affect offspring responses, with evidence for both positive and negative effects on offspring viability and tolerance to HypOA. 


Related Datasets

IsRelatedTo

Dataset: Atlantic silverside HypOA experiments: Larval morphometric measurements
Relationship Description: Data from the same experiments with the same individuals.
Murray, C. S. (2024) Morphological measurements of Atlantic silverside (Menidia menidia) larvae reared under ambient or hypoxia/acidification treatments during cross-generational laboratory experiments in 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-16 http://lod.bco-dmo.org/id/dataset/925042

Related Publications

Results

Murray, C. (2024) Results publication in preparation.
Methods

Dickson, A. G. (1990). Thermodynamics of the dissociation of boric acid in synthetic seawater from 273.15 to 318.15 K. Deep Sea Research Part A. Oceanographic Research Papers, 37(5), 755–766. doi:10.1016/0198-0149(90)90004-f
Methods

Dickson, A. G., & Millero, F. J. (1987). A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep Sea Research Part A. Oceanographic Research Papers, 34(10), 1733–1743. doi:10.1016/0198-0149(87)90021-5
Methods

Dickson, A., National Oceanic and Atmospheric Administration (n.d.). CRM Batches: Information on Batches of CO2 in Seawater Reference Material. Ocean Carbon and Acidification Data System. Available from https://www.nodc.noaa.gov/ocads/oceans/Dickson_CRM/batches.html
Methods

Mehrbach, C., Culberson, C. H., Hawley, J. E., & Pytkowicx, R. M. (1973). Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure. Limnology and Oceanography, 18(6), 897–907. doi:10.4319/lo.1973.18.6.0897