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Dataset: HPLC and fluorometric derived phytoplankton pigment data from BATS Validation cruises for years 1996-2023

Preliminary and in progressVersion 4 (2024-05-03)Dataset Type:Cruise Results

Nicholas Bates (Principal Investigator)

Rodney J. Johnson (Co-Principal Investigator)

Paul J. Lethaby (Scientist)

Claire Medley (Scientist)

Dominic Smith (Data Manager)


Program: Ocean Carbon and Biogeochemistry (OCB)

Program: U.S. Joint Global Ocean Flux Study (U.S. JGOFS)

Program: Ocean Time-series Sites (Ocean Time-series)

Project: Bermuda Atlantic Time-series Study (BATS)


Abstract

Data presented here are discrete bottle HPLC derived phytoplankton pigments and fluorometric chlorophyll-a for BATS validation (BVAL) cruises from June 1996 (BVAL cruise #16) through June 2023 (BVAL cruise #60). Water samples are typically collected from 7-12 depths in the upper 250 meters of the water column, and then filtered under low vacuum through a 25mm GF/F filter. The filter is then flash frozen in liquid nitrogen and stored at -80 degrees C. Shoreside, analysis is performed on an HPLC u...

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Following the first several years of the BATS project it was deemed necessary by the JGOFS steering committee and BATS PI's to conduct validation cruises in the vicinity of the nominal BATS site to better understand the mesoscale and larger scale variability of the region. In particular, a focus of the BVAL cruises was to assess the spatial scale representation of the BATS and Hydrostation ‘S’ programs. Initial focus of the BVAL cruises was to investigate mesoscale variability and meridional gradients of the local region. Later, cruises focused on specific mesoscale eddies (e.g., McGillicuddy et al., 1998; McGillicuddy et al., 1999) and effects of tropical cyclones through the local region. In the year 2000 it was deemed more important to document the larger scale changes in the North Atlantic Subtropical gyre so BVAL cruises established a transect line from ~ 35N to 19N (Bermuda to Puerto Rico) very similar to the WOCE A22 repeat hydrography line (Johnson et al., 2020). These annual Bermuda to Puerto Rico transects have been run since 2000 and target stations at every one degree of latitude and typically have been conducted in September/October of each year to capture maximal heat content in the upper ocean. However, since this timeframe coincides with high tropical cyclone activity the cruises were reluctantly (as of 2022) moved to begin in June/July of each year for safety and operational reasons. In the pentad prior to 2022 every BVAL cruise was significantly impacted by multiple tropical cyclones. Additionally, some BVAL cruises are transects from the US east coast to Bermuda and are opportunistic cruises leveraging times when the BIOS resident vessel (R/V Weatherbird II or R/V Atlantic Explorer) is in transit for other research cruises or ship yard visits. Parameters presented are the same as provided in the standard BATS bottle files. To browse cruise tracks please see the supplemental information.

 


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Related Publications

Methods

Bermuda Atlantic Time-series Study Methods (online at https://bats.bios.edu/about/cruise-information/)
Methods

Bidigare, R. R., Van Heukelem, L., & Trees, C. C. (2002). HPLC phytoplankton pigments: sampling, laboratory methods, and quality assurance procedures. Ocean optics protocols for satellite ocean color sensor validation, revision, 3(2), 258-268.
Methods

Bidigare, R. R., Van Heukelem, L., & Trees, C. C. (2005). Analysis of Algal Pigments by High-Performance Liquid Chromatography. Algal Culturing Techniques, 327–345. https://doi.org/10.1016/b978-012088426-1/50021-4
Methods

Bidigare, R.R., 1991. Analysis of algal chlorophylls and carotenoids. In: D.C. Hurd and D.W. Spencer (Editors), Marine Particles: Analysis and Characterization. Am. Geophys. Union, Washington, DC, pp. 119-123.
Methods

Grasshoff, K., Kremling, K., & Ehrhardt, M. (Eds.). (1999). Methods of Seawater Analysis. doi:10.1002/9783527613984