©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.Algal photosynthetic metabolism changes depending on light availability. These metabolic shifts alter how photosynthetic energy is allocated into different biosynthetic and catabolic pathways yielding different metabolic intermediates and end products. Our research focuses on the metabolic intermediates and end products that are volatile organic compounds (VOCs). This dataset gives the full range of VOCs produced by the diatom, Phaeodactylum tricornutum, and detected by proton transfer reaction ...
Show moreVolatile organic compounds (VOCs) were collected from axenic Phaeodactylum tricornutum (urn:lsid:marinespecies.org:taxname:175584) every 2 hours across a diel cycle using proton transfer reaction time-of-flight mass spectroscopy (PTR-TOF/MS; Ionicon, Austria) as previously described (Moore et al., 2020). Autoclaved f/2+Si media and HPLC-grade water were measured alongside the samples as blank (background) controls. 100 ml culture, media, or HPLC water was transferred to a dynamic stripping chamber (Halsey et al., 2017) and bubbled with breathing-grade air passed first through a hydrocarbon trap and then through a glass frit in the bottom of the chamber at 50 ml min-1 for 5 min to strip VOCs from the sample.
The stripped gasses were directed into the PTR-ToF/MS for measurement via soft ionization with H3O+. The mass spectrum (30-240 a.m.u.) was acquired at 5 s intervals.
PTR-TOF/MS data were analyzed using PTR viewer 3.4.3 (Ionicon Analytik).
Halsey, K. (2024) Results of diel cycle experiments including the full range of volatile organic compounds (VOCs) produced by the diatom P. tricornutum during laboratory experiments conduced in October of 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-05-21 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/928033 [access date]
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