Dataset: Thalassiosira pseudonana CCMP1335 endometabolite uptake by Ruegeria pomeroyi DSS-3

Final no updates expectedVersion 1 (2024-07-15)Dataset Type:experimental

Principal Investigator: Mary Ann Moran (University of Georgia)

Scientist: Malin Olofsson (University of Georgia)

Technician: Christa Smith (University of Georgia)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)


Project: Effects of Climate Change Variables on Microbial Autotroph-Heterotroph Carbon Flux (CC_Auto_Hetero_Fluxes)


Abstract

The ocean’s temperature increase has fundamental implications for physiological rates and processes of marine microbes. In this study, a marine diatom Thalassiosira pseudonana CCMP1335 was acclimated for three months at temperatures below (14°C), equal to (20°C), and above (28°C). Heterotrophic bacterium Ruegeria pomeroyi DSS-3 was inoculated into cultures, and transporter expression was compared between temperatures. R. pomeroyi transporter expression leveraged as a biosensor of available dia...

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All the raw spectra, NMRPipe scripts for spectrum processing, and MATLAB scripts for data analysis are deposited in Metabolomics Workbench under Project ID PR001837 (see related datasets).

 


Related Datasets

IsRelatedTo

Dataset: https://doi.org/10.46936/10.25585/60001361
Moran, M.A., Hopkinson, B. Olofsson, M., McKenzie, P., Uchimiya, M. Temperature Effects on Metabolite-Mediated Autotroph-Heterotroph Carbon Transfer. Joint Genome Institution. https://doi.org/10.46936/10.25585/60001361
IsRelatedTo

Dataset: https://doi.org/doi:10.21228/M88B0T
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org where it has been assigned Project ID PR001837. The data can be accessed directly via it's Project doi: 10.21228/M88B0T. This work is supported by Metabolomics Workbench/National Metabolomics Data Repository (NMDR) (grant# U2C-DK119886), Common Fund Data Ecosystem (CFDE) (grant# 3OT2OD030544) and Metabolomics Consortium Coordinating Center (M3C) (grant# 1U2C-DK119889)..

Related Publications

Results

Malin Olofsson, Mario Uchimiy, Frank X. Ferrer-González, Jeremy E. Schreier, McKenzie A. Powers, Christa B. Smith, Arthur S. Edison, and Mary Ann Moran. Metabolite Profiling of Temperature-Acclimated Marine Diatoms Co-cultured with Heterotrophic Bacteria. In Revision.