Dataset: Phosphate Profile Data Comparison between Programmable Flow Injection (pFI) and AA3 Autoanalyzer Methods from R/V Kilo Moana KM2210 in the North Pacific Subtropical Gyre during September 2022 (pFI-SI-LOV project)

This dataset has not been validatedPreliminary and in progressVersion 1 (2024-07-05)Dataset Type:Cruise Results

Principal Investigator: Maxime Grand (Moss Landing Marine Laboratories)

Student: Marine Lebrec (Moss Landing Marine Laboratories)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Developing Automated Nutrient and Trace Metal Methodology using Programmable Flow Injection (pFI-SI-LOV)


Abstract

These data represent a full depth profile collected at the Hawaii Ocean Time Series station ALOHA in September 2022. The pFI_PO4 column shows phosphate concentrations from unfiltered samples analyzed onboard the RV Kilo Moana using a programmable Flow Injection (pFI) analyzer, calibrated with phosphate standards prepared in Milli-Q water. The SIO_PO4 column contains data from replicate samples taken from the same depths. These samples were frozen, shipped on dry ice to the Scripps Institution of...

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We collected unfiltered seawater samples directly from the Niskin bottles. One set was analyzed in triplicate for phosphate using the pFI analyzer calibrated with MQ standards (0-3 µmol L-1). The duplicate samples were frozen, shipped to Scripps Institution of Oceanography (SIO) Ocean Data Facility chemistry laboratory on dry ice and analyzed at SIO’s lab using an AA3 autoanalyzer. 

The pFI analyzer was calibrated with working phosphate standards prepared in MQ water (0-3 µmol L-1). To calculate phosphate concentrations at each depth, we took the mean of the baseline corrected absorbance at 880 nm minus the reference absorbance at 1050 nm. Phosphate concentrations were then calculated using the slope and intercept of the MQ calibration curve. 

pFI LOD: 0.06 umol/L

pFI precision: 4.1%


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Results

Lebrec, M., & Grand, M. M. (2024). Programmable flow injection: a versatile technique for benchtop and autonomous analysis of phosphate and silicate in seawater. Frontiers in Marine Science, 11. https://doi.org/10.3389/fmars.2024.1354780