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Dataset: Programmable Flow Injection (pFI) Silicate Vertical Profile Data from R/V Investigator IN2023_V04 in the Southern Ocean during June 2023 (pFI-SI-LOV project)

Preliminary and in progressVersion 1 (2024-07-08)Dataset Type:Cruise Results

Maxime Grand (Principal Investigator, Contact)

Sawyer Newman (BCO-DMO Data Manager)


Project: Collaborative Research: Developing Automated Nutrient and Trace Metal Methodology using Programmable Flow Injection (pFI-SI-LOV)


Abstract

To establish the shipboard capabilities of our newly developed programmable Flow Injection (pFI) silicate analyzer, we collected and analyzed a full-depth vertical profile for silicate (Si) at the Southern Ocean Time Series in the Australian sector of the Southern Ocean in June 2023. For maximum accuracy, the pFI instrument was calibrated using working standards prepared in low nutrient seawater (LNSW, 0-119 umol/L). All 35 individual samples were analyzed in triplicate, the Si(OH)4 data lists ...

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Unfiltered seawater samples were collected from the Niskin bottles in acid-washed plastic tubes. The stored samples were immediately analyzed for silicate in triplicate using the pFI assay protocol outlined in Hatta et al. (2021). One key modification was adjusting the holding coils’ temperatures to 25ºC instead of the originally specified 50ºC. In tandem with this temperature change, the stop flow time in the flow cell was extended to 60 seconds to ensure the absorbance of the silicomolybdenum blue at 810 and 660 nm product reached a steady state. 

We used a miniSIA-2 instrument (miniSIA2, GlobalFIA, Fox Island, USA) equipped with a 8-port LOV unit and a 20 cm flow cell furnished with a Tungsten Halogen light source and UV-VIS spectrophotometer (Ocean Insight, USA) and a 14 position auto sampler (GlobalFIA, USA). The entire system was controlled with FloZF software (v 5.2, GlobalFIA). The system was calibrated using silicate working standards prepared in low nutrient seawater (LNSW, (0-119 µmol L-1 Si)). 


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Results

Lebrec, M., & Grand, M. M. (2024). Programmable flow injection: a versatile technique for benchtop and autonomous analysis of phosphate and silicate in seawater. Frontiers in Marine Science, 11. https://doi.org/10.3389/fmars.2024.1354780
Methods

Hatta, M., Ruzicka, J. (Jarda), Measures, C. I., & Davis, M. (2021). Programmable flow injection in batch mode: Determination of nutrients in sea water by using a single, salinity independent calibration line, obtained with standards prepared in distilled water. Talanta, 232, 122354. https://doi.org/10.1016/j.talanta.2021.122354
Methods

Truesdale, V. W., & Smith, C. J. (1976). The automatic determination of silicate dissolved in natural fresh water by means of procedures involving the use of either α- or β-molybdosilicic acid. The Analyst, 101(1198), 19. https://doi.org/10.1039/an9760100019