We conducted a preliminary study of the larval settlement success for the ascidian Phallusia nigra in response to temperature. Adult ascidians were collected from two locations and transported to the campus of NYU-Abu Dhabi. Gametes were collected from adults and embryos were fertilized in the laboratory. We quantified the number that metamorphosed and settled over a 92 hours.
Adult Phallusia nigra (LSID=urn:lsid:marinespecies.org:taxname:103725 ) were collected from two reef locations: Abu Dhabi and Fujairah. The adults were transferred to NYU-Abu Dhabi. Gametes were collected from adults by pipette and combined to produce embryos. Embryos (n=30) were then aliquoted into 6-well plates and cultured at three temperatures (27, 33 and 36 degree Celsius). Number of larvae that successfully metamorphosed and settled as juveniles were quantified at four time points (0, 8, 20, 44, and 92 hours).
Reitzel, A. (2024) Phallusia nigra larval settlement data from experiments with varying temperature conducted in May of 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-09-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.938160.1 [access date]
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