These are the raw data files from 5 cohorts of pacific herring embryos collected from Feb through April of 2022. Embryos were homogenized, and digested for proteomic analysis on a Lumos Orbitrap. Pooled samples were used with narrow window scans for library generation. The purpose of this data set is exploratory analysis for seasonal differences between early life stage proteome of pacific herring. All embryos are believed to be from the Semiahmoo Bay population since the Cherry Point popula...
Show moreEmbryos collected with a rake from small boat on 5 dates across three months and kept in a cooler with ambient seawater for 1-5 hours until returned to the lab. 1-5 embryos flash frozen and stored cyogenically for 3-6 months until processing.
Embryos were physically broken with a micropestle in a microcentriguge tube, then processed 3x 30 seconds with stainless steel beads in a bullet blender. Homogenate was removed from the bead then sonicated 3x. and digested using trypsin and a standard s-trap protocol. Tubes were dried using a speed vap and reconstituted in water/ACN.
Samples were spiked with Pierce retention time standards and with yeast enolase as a standard in some cases. A 90 minute gradient was run on the LC serving the Lumos.
(2024) Year 1 orbitrap MZML files. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-09-26 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/938909 [access date]
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