Dataset: Protist carbon from microscopy samples collected in the Argo Basin of the Eastern Indian Ocean on R/V Roger Revelle cruise RR2202 from Feb to Mar 2022

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.944892.1Version 1 (2024-12-03)Dataset Type:Cruise ResultsDataset Type:Other Field Results

Principal Investigator: Michael R. Landry (University of California-San Diego Scripps)

Co-Principal Investigator: Michael R. Stukel (Florida State University)

Scientist: Natalia Yingling (Florida State University)

BCO-DMO Data Manager: Audrey Mickle (Woods Hole Oceanographic Institution)


Program: Second International Indian Ocean Expedition (IIOE-2)

Project: Collaborative Research: Mesoscale variability in nitrogen sources and food-web dynamics supporting larval southern bluefin tuna in the eastern Indian Ocean (BLOOFINZ-IO)


Abstract

This dataset is from CTD-based water collections of samples for phytoplankton in the Argo Basin in the Eastern Indian Ocean aboard the R/V Roger Revelle cruise in Feb-March 2022 led by Dr. Michael Landry to investigate the plankton community composition and impacts on growth and survival of larval Southern Bluefin Tuna (SBT). These samples were stained and fixed for epifluorescence microscopy analysis. The microscopy results include the abundance and carbon-based biomass estimates of nano (2 to ...

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Field Collection

We conducted four Lagrangian experiments (hereafter referred to as “cycles”) that lasted three to five days.  The mixed layer was followed in a Lagrangian frame of reference using subsurface drogues attached to satellite-tagged marker buoys (Landry et al. 2009; Stukel et al. 2015). Six depths were chosen to span the euphotic zone (based on chlorophyll fluorescence measured during the conductivity-temperature-depth (CTD) downcast profiles).We used a 12-place 6-L Niskin bottle rosette equipped with a Seabird Conductivity, Temperature, and Depth (CTD) sensor and profiling chlorophyll fluorometer to sample water daily at six depths spanning the euphotic zone for phytoplankton community samples.

Epifluorescence Microscopy Sampling

From each depth, two different volumes of water were sampled: 50 mL for nanoplankton- epifluorescence microscopy (filtered through a 0.8-µm pore-size black polycarbonate filter) and 400 mL for microplankton epifluorescence microscopy (filtered through an 8-µm pore-size filter). Utilizing two different sized filters and sampling volumes allowed for appropriate, adjustable filtered volumes and avoid overlapping cells on the slides. 20 µm backing filters were utilized as data has indicated that they support the membrane filters and ensure even dispersal of sample on the filter (Kemp et. al., 1993; Taylor et al., 2015). The samples were preserved using buffered formalin, alkaline Lugol’s solution, and sodium thiosulfate then stained using proflavine and 4’, 6-diamidino-2phenylindole (DAPI) (modified protocol from Sherr and Sherr, 1993 in Kemp et. al., 1993). During and immediately after filtration, filters were covered with tin foil to prevent photochemical quenching. Filters were mounted onto a glass slide and frozen in a -80º C freezer for later analysis.


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Related Publications

General

Landry, M. R. (2025). R/V Roger Revelle Cruise RR2201 BLOOFINZ-IO Cruise Report. Eastern Indian Ocean larval tuna habitat study 20 January – 14 March 2022. http://hdl.handle.net/1834/43464
Methods

Kemp, P., B. F. Sherr, E. B. Sherr, and J. J. Cole (Eds.). (1993). Handbook of methods in aquatic microbial ecology. Lewis Publishing, Boca Raton, FL 33431, 777 pp.
Methods

Landry, M. R., Ohman, M. D., Goericke, R., Stukel, M. R., & Tsyrklevich, K. (2009). Lagrangian studies of phytoplankton growth and grazing relationships in a coastal upwelling ecosystem off Southern California. Progress in Oceanography, 83(1-4), 208–216. doi:10.1016/j.pocean.2009.07.026
Methods

Menden-Deuer, S., & Lessard, E. J. (2000). Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnology and Oceanography, 45(3), 569–579. doi:10.4319/lo.2000.45.3.0569
Methods

Sherr, B. F. and E. B. Sherr (1993) Preservation and storage of samples for enumeration of heterotrophic protists. In Kemp, P. F., Sherr, B. F., Sherr, E. B. and Cole, J. J. (eds.) Handbook of Methods in Aquatic Microbial Ecology. CRC Press, pp. 207-212