Dataset: Response of Bathycoccus sp. to iron-limitation sampled aboard R/V Roger Revelle RR1813 in the Subarctic North Pacific near Station PAPA (50 N, 144.8 W) from August to September 2018

Samples were taken at Ocean Station Papa, Subarctic North Pacific Ocean.  Sampling depth during cruise RR1813 was 3 m. The culture was isolated from a sample taken at approximate location (lat:50, lon:-145). Bathycoccus cells were isolated using a 10 mL pipette after whole seawater sampling from a trace metal clean rosette aboard the R/V Revelle.  Cultures were transported back to the University of North Carolina at Chapel Hill and stored in Aquil media under constant 120-150 µmol photons m-2 d-1 light in a 12ºC incubator.

Cultures were grown with either 1.37x10-6 M total Fe ("Fe+")  or 3.10x10-9 M total Fe ("Fe-") media.  Upon reaching exponential phase growth (based on trends in relative fluorescence units as measured by a Turner fluorometer),  cultures were filtered for chlorophyll a concentration, particulate carbon/particulate nitrogen, and RNA. Additional samples for cell count were collected and fixed with a 10% addition of paraformaldehyde. Measurements of the maximum quantum yield of photochemistry in photosystem II (Fv/Fm) and the functional absorption cross section of photosystem II (sigma) were collected. Filters used in chlorophyll and carbon/nitrogen sampling were uncombusted and combusted Whatman 0.7µm glass fiber filters, respectively.

For RNA sampling, Isopore 0.4 µm 47 mm polycarbonate filters were used. A RNAqueous-4PCR kit and RNeasy MinElute Clean-up kit were used for RNA extraction and clean-up.