Dataset: Invertebrate stable isotopes from samples collected at methane seeps off Southern California with the HOV Alvin from July 16-28, 2023 during R/V Atlantis cruise AT50-12

Puschore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Rocks were placed into individual compartments in a biobox on the Alvin basket. Before preserving, samples were kept cold and animals were handpicked to sample tissue for stable isotope analyses (see other data files for isotopic data). The tissue (aprox. 0.5-2.5 mg) was placed in pre weighted tin boats or combusted vials (500C overnight) and frozen at -70C. In addition to natural abundance analyses, some animals were placed into mylar bags and exposed to labeled C and N for incubation experiments. These were carried out for 3-8 days, and animals were then processed following the same method as for natural abundance samples. Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory.