Dataset: Bacteria Counts CTD Bottle Measurements from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022

This cruise visited eight stations on the Northeastern U.S. Continental Shelf. Latitudes and longitudes provided per sample in the data, but general station descriptions are below.

• Station 1 - Station 3: Georges Bank near the Gulf of Maine. Approximate location was 41.7 N, 68 W. Samples acquired from 0 m to 25 m depths.
• Station 4: The Great South Channel near the Gulf of Maine. Approximate location was 41.6 N, 69 W. Samples acquired from 0 m to 150 m depths.
• Station 5: Only one CTD profile was taken before this station was aborted due to weather. No data acquired. Station location was 40.8 N, 70.5 W.
• Station 6: Off the coast of Martha's Vineyard. Approximate location was 41.3 N, 70.5 W. Samples acquired from 0 m to 10 m depths.
• Station 7: At the mouth of the Sakonnet River near Newport, Rhode Island. Approximate location was 41.5 N, 71.2 W. Samples acquired from 0 m to 10 m depths.
• Station 8: Hudson Canyon near New York. Approximate location was 39.5 N, 72.3 W. Samples acquired from 0 m to 200 m depths.

Whole seawater and 1.2 um filtrates were fixed in final concentration of 0.5% glutaraldehyde for 15 min at 4 degrees, then flash frozen in liquid N2 and stored at -20 degree until analysis. Samples were thawed at room temperature and 10 ug/ml Tween-80 (final concentration) was added to each. Samples were sonicated for 1 min in a water bath (50W). A 20,000-fold dilution of Sybr Green I Nucleic Acid stain (Thermo-Fisher) was made in 0.2 um filtered 1XTE. 5ul of sample was mixed with 245 ul of 1X TE/Sybr Green I in a 96 well microtiter plate. Samples were counted on an Accrui C6 Plus flow cytometer on a fast flow rate, triggered off FL1-H<1000 for 30 sec.