Dataset: Metadata for RNASeq of green crabs collected in the northeast Pacific from Apr 2017 to Aug 2021

Final no updates expectedDOI: 10.26008/1912/bco-dmo.949682.1Version 1 (2025-01-28)Dataset Type:Other Field Results

Principal Investigator: Carolyn Tepolt (Woods Hole Oceanographic Institution)

Co-Principal Investigator: Emily Grason (Sea Grant)

Co-Principal Investigator: Patrick McDonald (University of Washington)

BCO-DMO Data Manager: Audrey Mickle (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Tracking fine-scale selection to temperature at the invasion front of a highly dispersive marine predator (West Coast Carcinus)


Abstract

This project explores genomic changes in the invasive European green crab (Carcinus maenas) in the northeast Pacific. It tracks the earliest stages of green crab invasion into a new environment where the species is predicted to have substantial ecological and economic impacts. Samples span time and space across the species range in the northeast Pacific, with a focus on areas where the species is currently expanding. This dataset includes individual metadata for 127 raw RNA-Seq reads, archived a...

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Samples of Carcinus maenas (urn:lsid:marinespecies.org:taxname:107381) were collected by large network of collaborators, usually by trapping but approaches vary. Heart tissue was dissected from crabs after severing the ventral nerve cord, and hearts were preserved in RNALater and frozen at -80 prior to extraction. Total RNA extraction was conducted with TRI reagent and 1-bromo-3-chloropropane. mRNA libraries were prepared with Illumina TruSeq Stranded mRNA kits and individually single-indexed with Illumina index sets A and B. Samples were multiplexed 24 / lane of 150 bp pe Illumina HiSeq4000 sequencing at Azenta (formerly Genewiz; South Plainfield NJ). 


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References

Dataset: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1170986
Woods Hole Oceanographic Institition. Carcinus maenas invasion in the northeast Pacific. 2024/10. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1170986. NCBI:BioProject: PRJNA1170986.

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