An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).
Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.
The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications.
Dataset Name | Brief Description |
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algal classes | ChemTax based chl-a of algal groups from the Sargasso Sea. |
Arabian Sea phytoplankton | Phytoplankton and nutrients from Northern Arabian Sea, 2009-2011 |
BLASTp homology data | BLASTp homology data from genes obtained in samples collected on LMG1411. |
California Mussel Larvae Experiments | Carbonate chemistry, shell growth, and respiration data from laboratory experiments on California mussel larvae. |
Cell biovolumes | Biovolume data from samples obtained on LMG1411. |
Chemical Defenses-1: Flores et al. 2012 | Chemical Defenses-1: Flores et al. 2012 |
Chemical Defenses-2: Senft-Batoh et al. 2015 (L&O) | Chemical Defenses-2: Senft-Batoh et al. 2015 (L&O) |
Chemical Defenses-3: Senft-Batoh et al. 2015 (Harmful Algae) | Chemical Defenses-3: Senft-Batoh et al. 2015 (Harmful Algae) |
Chemical Defenses-4: Griffin et al. 2019 | Chemical Defenses-4: Griffin et al. 2019 |
Chemical Defenses-5: Park and Dam 2021 | Chemical Defenses-5: Park and Dam 2021 |
Chemotactic response of Vibrio alginolyticus | Chemotaxis of Vibrio alginolyticus to control/phage-infected Synechococcus exudates |
Chemotaxis of P. haloplanktis towards exudates of Synechoccocus | |
Chemotaxis of V. alginolyticus towards Synechococcus Cells | Chemotaxis of Vibrio alginolyticus towards live phage-infected/control Synechococcus cells |
ciliate abundance and biomass | Abundance and biomass of ciliates in the Sargasso Sea from inverted microscope counts. |
Copepod grazing | Copepod grazing at low-high pCO2 levels |
Cryptophyte Cell Volumes | |
CTD Data with Chemical and Biological Discrete Samples | CTD Data with Chemical and Biological Discrete Samples |
Diatom growth rates | Cell growth rates from samples obtained on LMG1411. |
Diatom reference sequences | Reference sequences, genes, and K0 numbers for sampled diatoms. |
diatom_abundance_Ant1 | Diatom abundances from Antarctic phyto and microzooplankton experiments |
Dynamic mode structure of active turbulence | Dynamic mode structure of active turbulence |
Herbivorous protist abundances under simultaneus manipulation of temperature and nutrients | |
Initial prey abundance and biomass: MEPS 2017 | Initial prey abundance and biomass: MEPS 2017 |
Isolate information | Isolate information on genes found in samples collected on LMG1411. |
Lagrangian structure and stretching in bacterial turbulence | Lagrangian Structure and Stretching in Bacterial Turbulence |
Malaspina Expedition Nutrients and Phytoplankton | Concentration of inorganic nutrients, primary productivity measurements and phytoplankton cell concentration |
Microbial Cellular Abundance Epifluorescent Microscopy | |
Microscopy Counts | Microscopy Counts |
microzoo_abundance_Ant1 | Microzooplankton abundance from temp and iron experiments on Antarctic phyto and microzooplankton |
microzoo_abund_NAtl | Abundance results from temperature and pCO2 experiments on North Atlantic microzooplankton. |
microzoo_epi_abund | Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy. |
microzoo_epi_biomass | Carbon biomass estimates of heterotrophic protists by size-class, based on epifluorescence microscopy. |
MMETSP locations and e-values | MMETSP location information on samples obtained on LMG1411. |
MVCO CARD-FISH hybridization count results | MVCO CARD-FISH hybridization count results |
Phillips_et_al_2014 - Experiment 1 day | Lab experiment to test effects of live coral from Porites lobata, Pocillopora sp., Porites rus, and Millepora on Caerasignum maximum larvae to after 24 h. |
Phillips_et_al_2014 - Experiment 3 minutes | Lab experiment to test effects of short-term exposure to live coral from corals, Porites lobata, Pocillopora sp., Porites rus, on Caerasignum maximum larvae. |
phytopl_epi_abund | Cell abundance estimates of eukaryotic phytoplankton by taxa and size-class, based on epifluorescence microscopy. |
phytopl_epi_biomass | Carbon biomass estimates of eukaryotic phytoplankton by taxa and size-class, based on epifluorescence microscopy. |
Presence and absence of iron and light-related functional genes | Protein information obtained from samples collected on LMG1411. |
Protist Carbon | Protist Carbon |
PUA Microzooplankton Biomass | PUA Microzooplankton Biomass |
RV Atlantic Explorer BATS BV50 Plankton cell abundances and APA | Plankton cell abundances and APA data. |
Sample Accession Numbers | Project accession and library information on each experimental sample |
Series 4: Aggregation of Thalassiosira weissflogii as a function of pCO2, temperature and bacteria: Aggregation Phase - Carbonate System + TEP | Series 4: Aggregation of Thalassiosira weissflogii as a function of pCO2, temperature and bacteria: Aggregation Phase - Carbonate System + TEP |
Series 4: Aggregation of Thalassiosira weissflogii as a function of pCO2, temperature and bacteria: Aggregation Phase - Sinking Velocity | Series 4: Aggregation of Thalassiosira weissflogii as a function of pCO2, temperature and bacteria: Aggregation Phase - Sinking Velocity |
Shotgun Proteomics of Pseudonitzschia multiseries Multi stress incubations. | |
Temperature and nutrient dependent phytoplankton growth and herbivorous protist grazing rates | |
Transcriptome statistics | Transcriptome statistics from samples obtained on LMG1411. |
Upwelling Experiment Discrete Raw Measurements | Treatment and replicate IDs, as well as discrete raw measurements made for the upwelling experiments |
Vitacopss: eukaryote abundance by taxon | Eukaryote plankton abundance and composition with nitrate and B vitamins from San Pedro Ocean Timeseries (SPOT), 2015 |